Transgenic mice containing a human heavy chain immunoglobulin gene fragment cloned in a yeast artificial chromosome
| Autor: | Gregory N. Weddell, Barbara E. Pearson, Carole G. Kurahara, Clive S. Woodhouse, Roanna M. Ueda, Paul W. Hollenbach, Robert M. Kay, Ted K. Choi, Jeanne F. Loring |
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| Rok vydání: | 1993 |
| Předmět: |
Yeast artificial chromosome
Immunoglobulin gene Genetically modified mouse Male congenital hereditary and neonatal diseases and abnormalities Transgene Recombinant Fusion Proteins Molecular Sequence Data chemical and pharmacologic phenomena Mice Transgenic Biology Transfection Mice hemic and lymphatic diseases Genetics Animals Humans Cloning Molecular Gene Selectable marker Gene Library Heavy-chain antibody Base Sequence Genes Immunoglobulin Chimera Genome Human Stem Cells fungi hemic and immune systems Embryonic stem cell Molecular biology Immunoglobulin M Liposomes biology.protein Female Chromosomes Fungal Immunoglobulin Heavy Chains |
| Zdroj: | Nature genetics. 4(2) |
| ISSN: | 1061-4036 |
| Popis: | We have developed a method for the introduction of yeast artificial chromosomes (YACs) into transgenic mice. An 85 kilobase (kb) fragment of the human heavy chain immunoglobulin gene was cloned as a YAC, and embryonic stem cell lines carrying intact, integrated YACs were derived by co–lipofection of the YAC with an unlinked selectable marker. Chimaeric founder animals were produced by blastocyst injection, and offspring transgenic for the YAC were obtained. Analysis of serum from these offspring for human heavy chain antibody subunits demonstrated expression of the YAC–borne immunoglobulin gene fragment. Co–lipofection may prove to be a highly–successful means of producing transgenic mice containing large gene fragments in YACs. |
| Databáze: | OpenAIRE |
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