Kappa-opioid receptors on lymphocytes of a human lymphocytic cell line: morphine-induced up-regulation as evidenced by competitive RT-PCR and indirect immunofluorescence
| Autor: | Shunji Suzuki, Ronald Y. Chuang, Jean M. Bidlack, Linda F. Chuang, Roy H. Doi |
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| Rok vydání: | 2001 |
| Předmět: |
Agonist
medicine.medical_specialty medicine.drug_class Narcotic Antagonists Immunology (+)-Naloxone Biology κ-opioid receptor Jurkat cells Cell Line Opioid receptor Internal medicine medicine Humans Immunology and Allergy Lymphocytes RNA Messenger Fluorescent Antibody Technique Indirect Receptor Endogenous opioid Pharmacology Morphine Reverse Transcriptase Polymerase Chain Reaction Receptors Opioid kappa 3 4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide (trans)-Isomer Analgesics Non-Narcotic Molecular biology Naltrexone Up-Regulation Analgesics Opioid Endocrinology Opioid medicine.drug |
| Zdroj: | International Immunopharmacology. 1:1733-1742 |
| ISSN: | 1567-5769 |
| Popis: | We have previously shown that classical brain-like kappa opioid receptors (KOR) are constitutively expressed in lymphocytic cells. including human CEM x174 T-B hybrid cells, Jurkat -T4 cells, human peripheral blood mononuclear cells (PBMC), human CD4+ cells and monkey PBMC (Biochem. Biophys. Res. Commun. 209 (1995) 1003). The present study further demonstrates that the KOR of lymphocytes are activated in the presence of extracellular morphine or U50,488H, a KOR selective agonist, and the activation causes an increase in the expression of KOR mRNA, as determined by a quantitative competitive Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) procedure. The observed agonist-induced KOR up-regulation was blocked by treating the cells with either naloxone (a KOR-partially selective antagonist) or nor-binaltorphimine (a KOR-selective antagonist). Up-regulation of lymphocytic KOR by morphine was also evidenced by flow cytometric analysis of phycoerythrin (PE) amplification of fluorescein isothiocyanate-conjugated arylacetamide labeling of the KOR. Although morphine binds primarily to mu-opioid receptors, together with the previously reported phenomenon that morphine modulation of immune functions also exists in mu-opioid receptor knockout mice, the present study confirms that opioids such as morphine may exert their effects through multiple opioid receptor types and that the effects of morphine or endogenous opioids on immune cells could not be simply adduced from the anticipated effects of a synthetic, selective opioid receptor ligand. |
| Databáze: | OpenAIRE |
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