Different expression pattern of human cytomegalovirus-encoded microRNAs in circulation from virus latency to reactivation
| Autor: | Chen-Yu Zhang, Cheng Wang, Yujie Zhong, Wanqing Zhou, Yuying Bian, Han Shen, Chunni Zhang, Junjun Wang, Meng Ding |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Human cytomegalovirus viruses Cytomegalovirus lcsh:Medicine Viremia Disease Serum microRNA General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Virus latency microRNA medicine Humans Clinical significance Latency (engineering) business.industry Research lcsh:R virus diseases General Medicine Switch biochemical phenomena metabolism and nutrition medicine.disease Reactivation Virus Latency Reverse transcription polymerase chain reaction MicroRNAs 030104 developmental biology HCMV DNA load 030220 oncology & carcinogenesis Cytomegalovirus Infections Immunology Latency RNA Viral business |
| Zdroj: | Journal of Translational Medicine, Vol 18, Iss 1, Pp 1-13 (2020) Journal of Translational Medicine |
| ISSN: | 1479-5876 |
| Popis: | BackgroundHuman cytomegalovirus (HCMV) is a beta-hersvirinae that has a high latent infection rate worldwide and can cause serious consequences in immunocompromised patients when reactivation; however, the mechanism of how HCMV convert from latent to reactivation has rarely been investigated. In the present study, we aimed to perform a comprehensive analysis of the HCMV-encoded microRNA (miRNA) profile in serum of patients upon HCMV reactivation from latency and to further evaluate its clinical significance for the disease monitoring and preventing usefulness.MethodsSerum samples from 59 viremia patients and 60 age-gender matched controls were enrolled in this study for screening and validation of different expression of HCMV miRNAs. Serum concentrations of 22 known HCMV miRNAs were determined by a hydrolysis probe-based stem-loop quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay. HCMV DNA was measured by quantitative real-time PCR (qPCR) with the whole blood sample. Serum HCMV IgG and IgM were assessed using enzyme linked immunosorbent assay (ELISA). Another 47 samples from 5 patients at different time points were collected to evaluate the monitoring effectiveness and disease prediction ability of differential expression HCMV-miRNAs during the antiviral treatment.ResultsThe RT-qPCR analysis revealed that the serum levels of 16 of the 22 examined HCMV miRNAs were elevated in HCMV viremia patients compared with controls, and a profile of 8 HCMV miRNAs including hcmv-miR-US25-2-3p, hcmv-miR-US4-5p, hcmv-miR-US25-2-5p, hcmv-miR-US25-1-3p, hcmv-miR-US25-1, hcmv-miR-UL36, hcmv-miR-UL148D, hcmv-miR-US29-3p were markedly elevated (fold change > 2, P ConclusionsHCMV miRNAs profile showed markedly shift-switch from latency to reactivation in circulation from HCMV infected patients and hcmv-miR-US25-1-3p may be served as a predictor for the switch upon reactivation from latency in patients suffered with autoimmune diseases. |
| Databáze: | OpenAIRE |
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