Disease Outcome May Be Predicted by Molecular Detection of Minimal Residual Disease in Bone Marrow in Advanced Neuroblastoma: A Pilot Study
| Autor: | Keizo Horibe, Yoshiko Miyashita, Yuji Miyajima, Minoru Fukuda |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty Pathology Neoplasm Residual Time Factors Tyrosine 3-Monooxygenase medicine.medical_treatment Adrenal Gland Neoplasms Antineoplastic Agents Bone Neoplasms Pilot Projects Disease Hematopoietic stem cell transplantation Mediastinal Neoplasms Gastroenterology Disease-Free Survival Neuroblastoma Bone Marrow Internal medicine Cytology Antineoplastic Combined Chemotherapy Protocols medicine Humans RNA Messenger Retroperitoneal Neoplasms Neoplasm Metastasis Child Chemotherapy Reverse Transcriptase Polymerase Chain Reaction business.industry Hematopoietic Stem Cell Transplantation Infant medicine.disease Minimal residual disease Treatment Outcome medicine.anatomical_structure El Niño Child Preschool Female Bone marrow business |
| Zdroj: | Journal of Pediatric Hematology/Oncology. 23:10-13 |
| ISSN: | 0192-8562 |
| DOI: | 10.1097/00043426-200101000-00004 |
| Popis: | Purpose This pilot study focussed on whether sequential molecular detection of minimal residual disease (MRD) in bone marrow (BM) could predict the outcome of patients with advanced neuroblastoma. Patients and Methods Bone marrow samples from 21 patients older than age 12 months with stage IV neuroblastoma were sequentially examined for tumor cell contamination by detecting tyrosine hydroxylase (TH) messenger ribonucleic acid (mRNA) using reverse transcription-polymerase chain reaction (RT-PCR). Twenty patients received myeloablative therapy with hematopoietic stem cell transplantation after achieving complete remission. Results All BM samples of patients except that of one patient was cytologically positive for neuroblastoma cells at diagnosis, and they became negative for neuroblastoma cells within 3 months by cytologic examination. By RT-PCR analysis, BM samples of all patients were positive for TH mRNA at diagnosis, and samples of 19 patients became negative for TH mRNA 1 to 13 months after the start of chemotherapy. Six patients whose BM samples became negative for TH mRNA within 4 months after the start of chemotherapy remained alive without evidence of disease (median 76 mos, range 36[ndash]91). In contrast, out of 15 patients whose BM samples remained positive, 10 patients had relapse develop and 9 patients died from disease (median 15 mos, range 10[ndash]25). There was a statistically significant difference in disease-free survival between the two groups (P [lt] 0.05). Conclusion Persistence of MRD in BM may predict poor prognosis in advanced neuroblastoma. |
| Databáze: | OpenAIRE |
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