Protective effects of aerosolized pulmonary surfactant powder in a model of ventilator-induced lung injury
| Autor: | Yuqing Ye, Derek Daniher, James F. Lewis, Ruud A. W. Veldhuizen, Yingliang Ma, Jesse Zhu, Lynda McCaig |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Ventilator circuit Ventilator-Induced Lung Injury medicine.medical_treatment Pharmaceutical Science 02 engineering and technology Lung injury Protective Agents 030226 pharmacology & pharmacy Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Pulmonary surfactant medicine Animals Lung Saline Aerosolization Aerosols Mechanical ventilation Chemistry Pulmonary Surfactants respiratory system 021001 nanoscience & nanotechnology Dry-powder inhaler Disease Models Animal Anesthesia Breathing Powders Peptides 0210 nano-technology |
| Zdroj: | International Journal of Pharmaceutics. 583:119359 |
| ISSN: | 0378-5173 |
| DOI: | 10.1016/j.ijpharm.2020.119359 |
| Popis: | Mechanical ventilation may contribute to the impairment of the pulmonary surfactant system, which is one of the mechanisms leading to the progression of acute lung injury. To investigate the potential protective effects of pulmonary surfactant in a rat model of ventilator-induced lung injury, the surfactant powder was aerosolized using an in-house made device designed to deliver the aerosolized powder to the inspiratory line of a rodent ventilator circuit. Rats were randomized to (i) administration of aerosolized recombinant surfactant protein C based pulmonary surfactant, (ii) intratracheally instillation of the same surfactant re-constituted in saline, and (iii) no treatment. Animals were monitored during 2 h of high-tidal volume mechanical ventilation, after which rats were sacrificed, and further analysis of lung mechanics and surfactant function were completed. Blood gas measurements during ventilation showed extended maintenance of oxygen levels above 400 mmHg in aerosol treated animals over non-treated and instilled groups, while total protein analysis showed reduced levels in the aerosol compared to non-treated groups. Dynamic captive bubble surface tension measurements showed the activity of surfactant recovered from aerosol treated animals is maintained below 1 mN/m. The prophylactic treatment of aerosolized surfactant powder reduced the severity of lung injury in this model. |
| Databáze: | OpenAIRE |
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