N-(carboxymethyl)valine residues in hemoglobin (CMV-Hb) reflect accumulation of oxidative stress in diabetic patients
| Autor: | Tomoaki Shimizu, Satoshi Shimada, Hirotaka Watada, Chie Ohmura, Yoshifumi Tamura, Takahisa Hirose, Ryuzo Kawamori, Hiroshi Uchino, Yasushi Tanaka, Shinya Miwa, Tomiko Nakaniwa |
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| Rok vydání: | 2004 |
| Předmět: |
Glycation End Products
Advanced Male medicine.medical_specialty Antioxidant Endocrinology Diabetes and Metabolism medicine.medical_treatment medicine.disease_cause Hemoglobins Endocrinology In vivo Valine Glycation Diabetes mellitus Internal medicine Internal Medicine medicine Humans chemistry.chemical_classification Glycated Hemoglobin Reactive oxygen species business.industry virus diseases General Medicine Middle Aged medicine.disease Oxidative Stress Biochemistry chemistry Diabetes Mellitus Type 2 Female Hemoglobin business Reactive Oxygen Species Oxidative stress |
| Zdroj: | Diabetes research and clinical practice. 69(3) |
| ISSN: | 0168-8227 |
| Popis: | While carboxymethylated proteins are considered to be advanced glycation end products (AGE), they can also be induced by reactive oxygen species (ROS) independently of the AGE-forming process. To clarify whether N -(carboxymethyl)valine residues in hemoglobin (CMV-Hb) were a useful marker of the accumulation of ROS in diabetes, we evaluated CMV-Hb formation in vitro and in vivo. For the in vitro studying, purified human hemoglobin was incubated with d -glucose or d -glucose and hydrogen peroxide. For the in vivo study, CMV-Hb was extracted from peripheral red blood cells in diabetic patients and compared with that from non-diabetic subjects. Furthermore, the effect of antioxidants was evaluated after 6 months in 15 diabetic patients showing very high CMV-Hb levels. In vitro CMV-Hb formation increased in a glucose concentration and time-dependent manner. Co-incubation with glucose and hydrogen peroxide synergistically increased CMV-Hb formation. The CMV-Hb level was higher in the diabetic group than the non-diabetic group, and CMV-Hb was correlated with the plasma total cholesterol and serum creatinine levels. The CMV-Hb level was decreased by antioxidant therapy, whereas HbA1c did not change. These results demonstrate that CMV-Hb may be a useful marker for accumulation of oxidative stress in diabetic patients. |
| Databáze: | OpenAIRE |
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