Single cell oil production by a novel yeast Trichosporon mycotoxinivorans for complete and ecofriendly valorization of paddy straw
Autor: | Pawan Kumar Singh Nain, Shivani Chaturvedi, Anamika Sharma, Lata Nain, Sajish Sagia, Surender Singh |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine biology lcsh:Biotechnology Xylose Straw biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology Yeast Hydrolysate 03 medical and health sciences chemistry.chemical_compound Oleic acid 030104 developmental biology chemistry lcsh:Biology (General) 010608 biotechnology lcsh:TP248.13-248.65 Yeast extract Fermentation Food science Trichosporon mycotoxinivorans lcsh:QH301-705.5 Biotechnology |
Zdroj: | Electronic Journal of Biotechnology, Vol 44, Iss, Pp 60-68 (2020) |
ISSN: | 0717-3458 |
Popis: | Background: Oleaginous yeasts can be grown on different carbon sources, including lignocellulosic hydrolysate containing a mixture of glucose and xylose. However, not all yeast strains can utilize both the sugars for lipogenesis. Therefore, in this study, efforts were made to isolate dual sugar-utilizing oleaginous yeasts from different sources. Results: A total of eleven isolates were obtained, which were screened for their ability to utilize various carbohydrates for lipogenesis. One promising yeast isolate Trichosporon mycotoxinivorans S2 was selected based on its capability to use a mixture of glucose and xylose and produce 44.86 ± 4.03% lipids, as well as its tolerance to fermentation inhibitors. In order to identify an inexpensive source of sugars, nondetoxified paddy straw hydrolysate (saccharified with cellulase), supplemented with 0.05% yeast extract, 0.18% peptone, and 0.04% MgSO4 was used for growth of the yeast, resulting in a yield of 5.17 g L−1 lipids with conversion productivity of 0.06 g L−1 h−1. Optimization of the levels of yeast extract, peptone, and MgSO4 for maximizing lipid production using Box–Behnken design led to an increase in lipid yield by 41.59%. FAME analysis of single cell oil revealed oleic acid (30.84%), palmitic acid (18.28%), and stearic acid (17.64%) as the major fatty acids. Conclusion: The fatty acid profile illustrates the potential of T. mycotoxinivorans S2 to produce single cell oil as a feedstock for biodiesel. Therefore, the present study also indicated the potential of selected yeast to develop a zero-waste process for the complete valorization of paddy straw hydrolysate without detoxification.How to cite: Sagia S, Sharma A, Singh S, et al. Single cell oil production by a novel yeast Trichosporon mycotoxinivorans for complete and ecofriendly valorization of paddy straw. Electronic Journal of Biotechnology 2020;44. https://doi.org/10.1016/j.ejbt.2020.01.009. Keywords: Biodiesel, Fatty acids, Lignocellulosic biomass, Lignocellulosic hydrolysate, Lipid production, Lipogenesis, Oleaginous yeast, Paddy straw, Single cell oil, Trichosporon mycotoxinivorans, Yeast |
Databáze: | OpenAIRE |
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