Induction of heme oxygenase-1 after hyperosmotic opening of the blood-brain barrier
| Autor: | Linda Noble, Jeremy D. Richmon, Frank R. Sharp, Motoki Sato, Nino Maida, Kazumasa Fukuda, S. Scott Panter, Marcelle Bergeron |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Immunocytochemistry Immunoblotting Fluorescent Antibody Technique Biology Blood–brain barrier Rats Sprague-Dawley chemistry.chemical_compound Osmotic Pressure medicine Animals Receptor Molecular Biology Heme Microglia Glial fibrillary acidic protein General Neuroscience Molecular biology Immunohistochemistry Rats Heme oxygenase medicine.anatomical_structure nervous system chemistry Blood-Brain Barrier Enzyme Induction Immunoglobulin G Heme Oxygenase (Decyclizing) biology.protein Neurology (clinical) Heme Oxygenase-1 Developmental Biology Astrocyte |
| Zdroj: | Brain research. 780(1) |
| ISSN: | 0006-8993 |
| Popis: | The induction of the stress protein heme oxygenase-1 (HO-1) was studied in the rat brain after intracarotid administration of hyperosmolar mannitol. HO-1 was immunolocalized in fixed sections of brain 24 h to 7 days after injection. Immunoglobulin G (IgG) was immunolocalized in adjacent sections to demonstrate areas of breakdown of the blood–brain barrier. Induction of HO-1 was also evaluated by Western immunoblots, performed at 24 h after the insult. Immunofluorescent double labelling with monoclonal antibodies to HO-1 and either glial fibrillary acidic protein or the complement C3bi receptor was used to determine if glia/macrophages expressed HO-1. There was pronounced, widespread induction of HO-1 in the ipsilateral hemisphere and cerebellum by 24 h both by immunocytochemistry and by Western blots. This induction was markedly attenuated at later times. HO-1 was induced in astrocytes and microglia/macrophages in the ipsilateral hemisphere. In addition, the protein was induced in Bergmann glia and scattered microglia/macrophages in the cerebellum. The mechanism of induction of HO-1 in glia after opening of the blood–brain barrier could include exposure to heme proteins, denatured proteins and other plasma constituents known to induce HO-1. This glial induction may reflect a protective response of these cells. |
| Databáze: | OpenAIRE |
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