Structure and function of axo-axonic inhibition
| Autor: | Russel Torres, Sven Dorkenwald, Nicholas L. Turner, Anirban Nandi, Ignacio Tartavull, Jonathan Zung, Aleksandar Zlateski, Shelby Suckow, Chris S. Jordan, Ran Lu, Sergiy Popovych, Adam Bleckert, Costas A. Anastassiou, Dodam Ih, Agnes L. Bodor, Thomas Macrina, R. Clay Reid, Jun Zhuang, H. Sebastian Seung, Brian Hu, JoAnn Buchanan, Emmanouil Froudarakis, Andreas S. Tolias, Kisuk Lee, William Wong, Derrick Brittain, Forrest Collman, Thomas Chartrand, William Silversmith, Marc Takeno, Nico Kemnitz, Gayathri Mahalingam, Daniel J. Bumbarger, Lynne Becker, Jacob Reimer, Jingpeng Wu, Casey M Schneider-Mizell, Nuno Maçarico da Costa, Yang Li, Manuel Castro |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Mouse Interneuron QH301-705.5 Science Population Chandelier axon initial segment General Biochemistry Genetics and Molecular Biology Synapse Mice Calcium imaging Microscopy Electron Transmission medicine Animals Biology (General) visual cortex connectomics education education.field_of_study General Immunology and Microbiology Chandelier cell Chemistry Pyramidal Cells General Neuroscience General Medicine inhibition Visual cortex medicine.anatomical_structure Synapses Medicine Female Neuron Neuroscience Research Article |
| Zdroj: | eLife eLife, Vol 10 (2021) |
| ISSN: | 2050-084X |
| Popis: | Inhibitory neurons in mammalian cortex exhibit diverse physiological, morphological, molecular, and connectivity signatures. While considerable work has measured the average connectivity of several interneuron classes, there remains a fundamental lack of understanding of the connectivity distribution of distinct inhibitory cell types with synaptic resolution, how it relates to properties of target cells, and how it affects function. Here, we used large-scale electron microscopy and functional imaging to address these questions for chandelier cells in layer 2/3 of the mouse visual cortex. With dense reconstructions from electron microscopy, we mapped the complete chandelier input onto 153 pyramidal neurons. We found that synapse number is highly variable across the population and is correlated with several structural features of the target neuron. This variability in the number of axo-axonic ChC synapses is higher than the variability seen in perisomatic inhibition. Biophysical simulations show that the observed pattern of axo-axonic inhibition is particularly effective in controlling excitatory output when excitation and inhibition are co-active. Finally, we measured chandelier cell activity in awake animals using a cell-type-specific calcium imaging approach and saw highly correlated activity across chandelier cells. In the same experiments, in vivo chandelier population activity correlated with pupil dilation, a proxy for arousal. Together, these results suggest that chandelier cells provide a circuit-wide signal whose strength is adjusted relative to the properties of target neurons. |
| Databáze: | OpenAIRE |
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