Molecular margin status after radical prostatectomy using glutathione S-transferase P1 (GSTP1) promoter hypermethylation
| Autor: | Maria Friedrich, Johann Jakob Wendler, Daniel Baumunk, Martin Schostak, Burkhard Jandrig, Jorn H Witt, Markus Porsch, Uwe-B. Liehr |
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| Rok vydání: | 2021 |
| Předmět: |
Biochemical recurrence
Male Surgical margin medicine.medical_specialty Fossa Urology medicine.medical_treatment GSTP1 Prostate cancer medicine Humans Lymph node Grading (tumors) Glutathione Transferase Prostatectomy biology business.industry Prostate Margins of Excision Prostatic Neoplasms Prostate-Specific Antigen biology.organism_classification medicine.disease medicine.anatomical_structure Glutathione S-Transferase pi Neoplasm Recurrence Local business |
| Zdroj: | BJU internationalReferences. 130(4) |
| ISSN: | 1464-410X |
| Popis: | OBJECTIVE To assess the potential for molecular staging in biopsies of the prostatic fossa after radical prostatectomy (RP) by searching for occult tumour cells through analysis of glutathione S-transferase P1 (GSTP1) methylation status. PATIENTS AND METHODS We analysed 2446 biopsies: 2286 biopsies from a group of 254 patients with clinically organ-confined prostate cancer who underwent RP and 160 biopsies from a control group of 32 patients. After prostate gland excision, biopsies were obtained from defined areas of the prostatic fossa and bisected for histopathological and molecular genetics analyses. Results were related to clinicopathological data including tumour stage, lymph node status, resection status, tumour grading, initial PSA level, and biochemical recurrence. RESULTS In total, 34 patients (13.4%) had at least one core positive for the GSTP1 promoter hypermethylation, six of whom (17.6%) were characterised as having a clinically localised tumour stage (pT2, pN0) and 28 (82.4%) as an advanced tumour stage (≥pT3 and/or pN1). GSTP1 promoter hypermethylation significantly correlated with tumour stage (P |
| Databáze: | OpenAIRE |
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