Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020

Autor: Victor V. Revin, Richard J. Roberts, Elena V Liyaskina, Alevtina A. Kitykina, Philip V. Toukach, Nadezhda A. Rakova, Saulius Vainauskas, Valentina V. Rusyaeva, Alexey Fomenkov
Rok vydání: 2021
Předmět:
0106 biological sciences
Sucrose
Exopolysaccharides
Magnetic Resonance Spectroscopy
Glycobiology
Disaccharides
Spectrum analysis techniques
Fructoses
Biochemistry
01 natural sciences
Epigenome
Spectroscopy
Fourier Transform Infrared
Glycoside hydrolase
Cloning
Molecular
0303 health sciences
Multidisciplinary
biology
Strain (chemistry)
Organic Compounds
Chemistry
Monosaccharides
Polysaccharides
Bacterial
Genomics
Nuclear magnetic resonance spectroscopy
Physical Sciences
Medicine
Gene Cloning
Paenibacillus polymyxa
Research Article
Science
Size-exclusion chromatography
Carbohydrates
03 medical and health sciences
Paenibacillus
NMR spectroscopy
Fructan
Polysaccharides
010608 biotechnology
Genetics
Molecular Biology Techniques
Molecular Biology
030304 developmental biology
Organic Chemistry
Chemical Compounds
Biology and Life Sciences
Spectrometry
X-Ray Emission
Levansucrase
Sequence Analysis
DNA
biology.organism_classification
Research and analysis methods
Glucose
Microscopy
Electron
Scanning
Cloning
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 7, p e0253482 (2021)
ISSN: 1932-6203
Popis: Paenibacillus spp. exopolysaccharides (EPSs) have become a growing interest recently as a source of biomaterials. In this study, we characterized Paenibacillus polymyxa 2020 strain, which produces a large quantity of EPS (up to 68 g/L),and was isolated from wasp honeycombs. Here we report its complete genome sequence and full methylome analysis detected by Pacific Biosciences SMRT sequencing. Moreover, bioinformatic analysis identified a putative levan synthetic operon. SacC and sacB genes have been cloned and their products identified as glycoside hydrolase and levansucrase respectively. The Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra demonstrated that the EPS is a linear β-(2→6)-linked fructan (levan). The structure and properties of levan polymer produced from sucrose and molasses were analyzed by FT-IR, NMR, scanning electron microscopy (SEM), high performance size exclusion chromatography (HPSEC), thermogravimetric analysis (TGA), cytotoxicity tests and showed low toxicity and high biocompatibility. Thus, P. polymyxa 2020 could be an exceptional cost-effective source for the industrial production of levan-type EPSs and to obtain functional biomaterials based on it for a broad range of applications, including bioengineering.
Databáze: OpenAIRE
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