Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by Engineered Escherichia coli
| Autor: | Dimitri Dascier, J. David Rozzell, Spiros Kambourakis, Ling Hua, Jon D. Stewart |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
Ketone Nicotinamide biology Regeneration (biology) Organic Chemistry medicine.disease_cause Combinatorial chemistry Article Cofactor chemistry.chemical_compound Enzyme chemistry medicine biology.protein Physical and Theoretical Chemistry Escherichia coli Acetophenone |
| Zdroj: | Organic Process Research & Development |
| ISSN: | 1520-586X 1083-6160 |
| Popis: | This study was designed to determine whether whole cells or crude enzyme extracts are more effective for preparative-scale ketone reductions by dehydrogenases as well as learning which cofactor regeneration scheme is most effective. Based on results from three representative ketone substrates (an α-fluoro-β-keto ester, a bis-trifluoromethylated acetophenone, and a symmetrical β-diketone), our results demonstrate that several nicotinamide cofactor regeneration strategies can be applied to preparative-scale dehydrogenase-catalyzed reactions successfully. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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