The influence of exogenous melatonin on antioxidative status in seminal plasma and spermatozoa in French Alpine bucks during the nonbreeding season
| Autor: | Dražen Đuričić, Branimira Špoljarić, Marko Samardžija, Jadranka Pejaković Hlede, Suzana Milinković-Tur, Velimir Berta, Marinko Vilić, Jelena Šuran, Hrvoje Valpotić, Ivona Žura Žaja, Silvijo Vince, Daniel Špoljarić |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty endocrine system Glutathione reductase Semen Antioxidants Gene Expression Regulation Enzymologic Superoxide dismutase Andrology Melatonin chemistry.chemical_compound Endocrinology Food Animals Internal medicine medicine Animals chemistry.chemical_classification Drug Implants Glutathione Peroxidase biology Superoxide Dismutase Glutathione peroxidase Goats Glutathione Malondialdehyde Catalase Spermatozoa Semen Analysis Glutathione Reductase chemistry biology.protein Animal Science and Zoology Exogenous melatonin Seminal plasma Ratios of antioxidative enzymes Goat Seasons medicine.drug |
| Popis: | Research data regarding exogenous melatonin effects on buck semen antioxidative status during the breeding and nonbreeding seasons are scarce or missing. The aim of this study was to investigate the effect of melatonin implants on the activity and ratios of antioxidative enzymes and on malondialdehyde concentrations in the seminal plasma and spermatozoa of French Alpine bucks during the 3 mo of the nonbreeding season. Two groups of randomly assigned bucks were either treated with melatonin (n = 6) or served as controls (n = 6). Semen samples were collected weekly, seminal plasma was separated from spermatozoa, and activities of glutathione peroxidase (GSH-Px), glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT), and the concentration of malondialdehyde were determined. The melatonin-treated bucks had significantly higher CAT/SOD and GSH-Px/SOD ratios, but a significantly lower GR/GSH-Px ratio in seminal plasma and spermatozoa during the different periods of the nonbreeding season. In conclusion, exogenous melatonin influenced the activities of all ratios and some single antioxidative enzymes tested in bucks' seminal plasma and spermatozoa. Here we provide the first evidence derived from an intervention, that is, melatonin treatment, which affects antioxidative enzymes ratios in the semen of bucks, and could thus be recommended as an indicator of oxidative stress severity and antioxidative status of semen. |
| Databáze: | OpenAIRE |
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