In vivo expression of interleukin-8, and regulated on activation, normal, T-cell expressed, and secreted, by human germinal centre B lymphocytes
| Autor: | Christiane Guret, Liliana Guzman-Rojas, Kelly Cain, Stephen E. Ullrich, Dat X. Nghiem, Jennifer Sims-Mourtada, Hector Martinez-Valdez, Roberto Rangel |
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| Rok vydání: | 2003 |
| Předmět: |
Chemokine
Receptors CCR5 T cell T-Lymphocytes Immunology Antigens CD19 Lymphocyte Cooperation B-Lymphocyte Subsets Lymphocyte Activation Receptors Interleukin-8A medicine Immunology and Allergy Humans CXC chemokine receptors Interleukin 8 Chemokine CCL5 CXCL16 biology Interleukin-8 Germinal center Interleukin Original Articles Germinal Center Molecular biology Chemotaxis Leukocyte medicine.anatomical_structure biology.protein Homing (hematopoietic) |
| Zdroj: | Immunology. 110(3) |
| ISSN: | 0019-2805 |
| Popis: | T-cell homing within germinal centres (GCs) is required for humoral B-cell responses. However, the mechanisms implicated in the recruitment of T cells into the GC are not completely understood. Here we show, by immunohistology, and Northern and Western blots, that in vivo human GC B lymphocytes can express CxC and CC chemokines. Moreover, B-cell subset-specific experiments reveal that interleukin (IL)-8 and regulated on activation, normal, T-cell expressed, and secreted (RANTES) are predominantly expressed by GC centroblast and centrocytes, suggesting that chemokine expression is essential at stages in which B-lymphocytes engage in active antigen-dependent interactions with T lymphocytes. In keeping with this hypothesis, we show that the T cells recruited into the GC correlatively express the receptors for IL-8 and RANTES. We propose that chemokine expression is a key B-cell function that facilitates T-lymphocyte recruitment into the GCs and supports cognate B-cell : T-cell encounters. Moreover, our data are consistent with the impaired homing of T cells to secondary lymphoid organs in mice that are either deficient in CC and CxC chemokines or their receptors. |
| Databáze: | OpenAIRE |
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