Dual-Color 3D Superresolution Microscopy by Combined Spectral-Demixing and Biplane Imaging
| Autor: | David Albrecht, Helge Ewers, Evgenia Platonova, Christian M. Winterflood |
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| Rok vydání: | 2015 |
| Předmět: |
Ankyrins
Point spread function Computer science Biophysics Color 02 engineering and technology Hippocampus Biplane Rats Sprague-Dawley 03 medical and health sciences Imaging Three-Dimensional Optics Cell Line Tumor Chromatic aberration Microscopy Image Processing Computer-Assisted Animals Humans Nanoscopic scale Cells Cultured Fluorescent Dyes 030304 developmental biology Neurons 0303 health sciences Biophysical Letter business.industry Optical Imaging Resolution (electron density) Spectrin Carbocyanines 021001 nanoscience & nanotechnology Superresolution 0210 nano-technology business Dual color |
| Zdroj: | Biophysical Journal. 109:3-6 |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/j.bpj.2015.05.026 |
| Popis: | Multicolor three-dimensional (3D) superresolution techniques allow important insight into the relative organization of cellular structures. While a number of innovative solutions have emerged, multicolor 3D techniques still face significant technical challenges. In this Letter we provide a straightforward approach to single-molecule localization microscopy imaging in three dimensions and two colors. We combine biplane imaging and spectral-demixing, which eliminates a number of problems, including color cross-talk, chromatic aberration effects, and problems with color registration. We present 3D dual-color images of nanoscopic structures in hippocampal neurons with a 3D compound resolution routinely achieved only in a single color. |
| Databáze: | OpenAIRE |
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