The Center for Eukaryotic Structural Genomics
| Autor: | Brian F. Volkman, Karl W. Nichols, Brian G. Fox, David J. Aceti, Frank C. Vojtik, Russell L. Wrobel, John L. Markley, George N. Phillips, Craig A. Bingman, John G. Primm, Zsolt Zolnai, Sarata C. Sahu, Ronnie O. Frederick, Shin-ichi Makino |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Proteomics
Protein Conformation 030303 biophysics Genomics Computational biology Crystallography X-Ray Biochemistry Article CESG Structural genomics 03 medical and health sciences PepcDB Protein structure Structural Biology Genetics TEV protease Protein production Animals Humans LIMS Protein Structure Initiative (PSI) Plasmid design Nuclear Magnetic Resonance Biomolecular 030304 developmental biology 0303 health sciences Multi-Institutional Systems biology Galdieria sulphuraria Proteins General Medicine biology.organism_classification Protein structure determination PSI Materials Repository Cyanidioschyzon merolae Protein Structure Initiative |
| Zdroj: | Journal of Structural and Functional Genomics |
| ISSN: | 1570-0267 1345-711X |
| Popis: | The Center for Eukaryotic Structural Genomics (CESG) is a “specialized” or “technology development” center supported by the Protein Structure Initiative (PSI). CESG’s mission is to develop improved methods for the high-throughput solution of structures from eukaryotic proteins, with a very strong weighting toward human proteins of biomedical relevance. During the first three years of PSI-2, CESG selected targets representing 601 proteins from Homo sapiens, 33 from mouse, 10 from rat, 139 from Galdieria sulphuraria, 35 from Arabidopsis thaliana, 96 from Cyanidioschyzon merolae, 80 from Plasmodium falciparum, 24 from yeast, and about 25 from other eukaryotes. Notably, 30% of all structures of human proteins solved by the PSI Centers were determined at CESG. Whereas eukaryotic proteins generally are considered to be much more challenging targets than prokaryotic proteins, the technology now in place at CESG yields success rates that are comparable to those of the large production centers that work primarily on prokaryotic proteins. We describe here the technological innovations that underlie CESG’s platforms for bioinformatics and laboratory information management, target selection, protein production, and structure determination by X-ray crystallography or NMR spectroscopy. |
| Databáze: | OpenAIRE |
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