Nested genetic bit analysis (N-GBA) for mutation detection in the p53 tumor suppressor gene
| Autor: | Stephen Anderson, Kalpana Parikh, Guang Lan, Steven R. Head, Michael T. Boyce-Jacino, Yu-Hui Rogers, Philip Goelet |
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| Rok vydání: | 1997 |
| Předmět: |
DNA Mutational Analysis
Adenocarcinoma Biology Polymerase Chain Reaction Primer extension law.invention Mice chemistry.chemical_compound Exon law Genetics Animals Humans Missense mutation Mutation detection Codon Gene Polymerase chain reaction DNA Primers Sequence Deletion DNA Neoplasm Templates Genetic Genes p53 Pancreatic Neoplasms Mutagenesis Insertional Biliary Tract Neoplasms chemistry DNA microarray Neoplasm Transplantation DNA Research Article |
| Zdroj: | Nucleic Acids Research. 25:5065-5071 |
| ISSN: | 1362-4962 |
| DOI: | 10.1093/nar/25.24.5065 |
| Popis: | There is a growing and significant demand for reliable, simple and sensitive methods for repeated scanning of a given gene or gene fragment for detection and characterization of mutations. Solid-phase sequencing by single base primer extension of nested GBATM primers on miniaturized DNA arrays can be used to effectively scan targeted sequences for missense, insertion and deletion mutations. This paper describes the use of N-GBA arrays designed to scan the sequence of a 33 base region of exon 8 of the p53 gene (codons 272-282) encompassing a hot spot for mutations associated with the development of cancer. Synthetic DNA templates containing various missense, insertion and deletion mutations, as well as DNA prepared from pancreatic and biliary tumor cells, were genotyped using the exon 8 arrays. |
| Databáze: | OpenAIRE |
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