The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A

Autor: Jonas Seiler, Annika Eiteneuer, Hemmo Meyer, Monica Gotta, René Holtackers, Françoise Schwager, Esther Zanin, François Prodon, Asako Sugimoto, Patrick Meraldi, Elsa Kress, Mika Toya
Rok vydání: 2013
Předmět:
Cell division
Medizin
Caenorhabditis elegans Proteins/metabolism/physiology
Mitosis
Centrosome cycle
Cell Cycle Proteins
Biology
Protein Serine-Threonine Kinases
Adaptor Proteins
Signal Transducing/metabolism/physiology
Article
Gene Expression Regulation
Enzymologic
Cell Line
03 medical and health sciences
0302 clinical medicine
Animals
Humans
Caenorhabditis elegans Proteins
ddc:612
Research Articles
030304 developmental biology
Adaptor Proteins
Signal Transducing
Green Fluorescent Proteins/metabolism
Adenosine Triphosphatases
Centrosome
0303 health sciences
Cell Cycle Proteins/metabolism
Cell Cycle
Gene Expression Regulation
Developmental
Cell Biology
Adenosine Triphosphatases/metabolism
Protein-Serine-Threonine Kinases/metabolism
Cell cycle
Caenorhabditis elegans/cytology/genetics
Cell biology
Spindle apparatus
Spindle checkpoint
Centrosome/metabolism
RNA Interference
Centrosome separation
030217 neurology & neurosurgery
Zdroj: JOURNAL OF CELL BIOLOGY
The Journal of Cell Biology, Vol. 201, No 4 (2013) pp. 559-575
The Journal of Cell Biology
ISSN: 0021-9525
Popis: UBXN-2, a substrate adaptor of the AAA ATPase CDC-48/p97, is required to coordinate centrosome maturation timing with mitosis.
Coordination of cell cycle events in space and time is crucial to achieve a successful cell division. Here, we demonstrate that UBXN-2, a substrate adaptor of the AAA ATPase Cdc48/p97, is required to coordinate centrosome maturation timing with mitosis. In UBXN-2–depleted Caenorhabditis elegans embryos, centrosomes recruited more AIR-1 (Aurora A), matured precociously, and alignment of the mitotic spindle with the axis of polarity was impaired. UBXN-2 and CDC-48 coimmunoprecipitated with AIR-1 and the spindle alignment defect was partially rescued by co-depleting AIR-1, indicating that UBXN-2 controls these processes via AIR-1. Similarly, depletion in human cells of the UBXN-2 orthologues p37/p47 resulted in an accumulation of Aurora A at centrosomes and a delay in centrosome separation. The latter defect was also rescued by inhibiting Aurora A. We therefore postulate that the role of this adaptor in cell cycle regulation is conserved.
Databáze: OpenAIRE
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