PRDX5 as a novel binding partner in Nrf2-mediated NSCLC progression under oxidative stress
| Autor: | Qun Xue, Xinming Chen, Ben Li, Weizhang Xiao, Xiang Cao |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Models
Molecular Aging Lung Neoplasms NF-E2-Related Factor 2 Gene Expression Apoptosis NSCLC medicine.disease_cause environment and public health Small hairpin RNA Carcinoma Non-Small-Cell Lung Cell Line Tumor NAD(P)H Dehydrogenase (Quinone) medicine Humans nuclear factor-related factor 2 Lung cancer Cell Proliferation peroxiredoxin 5 chemistry.chemical_classification Reactive oxygen species business.industry PRDX5 Peroxiredoxins Cell Biology respiratory system medicine.disease respiratory tract diseases Oxidative Stress chemistry Cell culture Disease Progression Cancer research NAD+ kinase Reactive Oxygen Species Peroxiredoxin business Oxidation-Reduction Oxidative stress Protein Binding Signal Transduction Research Paper |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| Popis: | Non-small-cell lung cancer (NSCLC) is one of the most common malignant tumors in the world. Reactive oxidative species (ROS) and nuclear factor-related factor 2 (Nrf2) -antioxidant response element (ARE) signal pathway are known to play important roles in the development of NSCLC. In this study, we identified Peroxiredoxin 5 (PRDX5) as a novel binding partner for Nrf2. PRDX5 was significantly increased in human NSCLC specimens and cell lines. Nrf2 interacted with PRDX5 in H2O2-stimulated NCSLC cells, and the interaction promoted the expression of NAD(P)H: quinone oxidoreductase 1 (NQO1) protein in NSCLC cells. Further, high expression of Nrf2 and PRDX5 were associated with worsened prognosis in patients with NSCLC significantly. Moreover, animal studies showed that the growth of tumors treated with Nrf2 and PRDX5 shRNA was significantly lower than that of the other groups. All these data indicated that overexpressed PRDX5 in NSCLC promoted binding with Nrf2 and enhanced NQO1 expression and NSCLC development. Overall, our studies demonstrated that PRDX5 can be a novel binding partner of Nrf2 in promoting NCSLC development under oxidative stress and provide potential opportunity for improving NSCLC therapy. |
| Databáze: | OpenAIRE |
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