Pseudomonas synxantha 2-79 Transformed with Pyrrolnitrin Biosynthesis Genes Has Improved Biocontrol Activity Against Soilborne Pathogens of Wheat and Canola

Autor:	Mingming Yang, Jason Kelton, David M. Weller, Olga V. Mavrodi, Jibin Zhang, Dmitri V. Mavrodi, Linda S. Thomashow
Rok vydání:	2020
Předmět:	biology Sclerotinia sclerotiorum food and beverages Plant Science biology.organism_classification Rhizoctonia Article Pythium ultimum Rhizoctonia solani Pseudomonas synxantha Horticulture Fusarium culmorum Root rot Stem rot Agronomy and Crop Science
Zdroj:	Phytopathology
ISSN:	1943-7684 0031-949X
Popis:	A four-gene operon (prnABCD) from Pseudomonas protegens Pf-5 encoding the biosynthesis of the antibiotic pyrronitrin was introduced into P. synxantha (formerly P. fluorescens) 2-79, an aggressive root colonizer of both dryland and irrigated wheat roots that naturally produces the antibiotic phenazine-1-carboxylic acid and suppresses both take-all and Rhizoctonia root rot of wheat. Recombinant strains ZHW15 and ZHW25 produced both antibiotics and maintained population sizes in the rhizosphere of wheat that were comparable to those of strain 2-79. The recombinant strains inhibited in vitro the wheat pathogens Rhizoctonia solani anastomosis group 8 (AG-8) and AG-2-1, Gaeumannomyces graminis var. tritici, Sclerotinia sclerotiorum, Fusarium culmorum, and F. pseudograminearum significantly more than did strain 2-79. Both the wild-type and recombinant strains were equally inhibitory of Pythium ultimum. When applied as a seed treatment, the recombinant strains suppressed take-all, Rhizoctonia root rot of wheat, and Rhizoctonia root and stem rot of canola significantly better than did wild-type strain 2-79.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::957420f2c205b164602f7d3be8d5324e https://doi.org/10.1094/phyto-09-19-0367-r Zobrazit plný text záznamu