Glycerol and neutral lipid production in the oleaginous marine diatom Phaeodactylum tricornutum promoted by overexpression of glycerol-3-phosphate dehydrogenase
| Autor: | Ying-Fang Niu, Wei-Dong Yang, Hong-Ye Li, Wei-Hong Xie, Yang Lu, Kun-Tao Peng, Yao Yao, Tan Huang, Jie-Sheng Liu |
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| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
biology Renewable Energy Sustainability and the Environment Fatty acid Management Monitoring Policy and Law biology.organism_classification Applied Microbiology and Biotechnology Metabolic engineering chemistry.chemical_compound General Energy Glycerol-3-phosphate dehydrogenase chemistry Biochemistry Biosynthesis DHAP Glycerol Phaeodactylum tricornutum Biotechnology Dihydroxyacetone phosphate |
| Zdroj: | Biotechnology for Biofuels. 7 |
| ISSN: | 1754-6834 |
| DOI: | 10.1186/1754-6834-7-110 |
| Popis: | Microalgae are ideal raw materials for biodiesel and bioactive compounds. Glycerol-3-phosphate is formed from dihydroxyacetone phosphate (DHAP) through the glycolytic pathway catalyzed by glycerol-3-phosphate dehydrogenase (GPDH). GPDH was characterized in the marine diatom Phaeodactylum tricornutum. In the GPDH-overexpressing P. tricornutum cells, the glycerol concentration per cell in the transformed diatom increased by 6.8-fold compared with the wild type, indicating that the overexpression of GPDH promoted the conversion of DHAP to glycerol-3-phosphate. There was a 60% increase in neutral lipid content, reaching 39.7% of dry cell weight in transgenic cells in the stationary phase, despite a 20% decrease in cell concentration. Fatty acid profiling showed that the levels of 16- and 18-carbon monounsaturated fatty acids significantly increased. GPDH had a significant impact on numerous metabolic processes in diatom cells, including the biosynthesis of glycerol and neutral lipids. These findings are instructive for the metabolic engineering of microalgae for biofuel production. |
| Databáze: | OpenAIRE |
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