Determination of histamine in human plasma: the European external quality control study 1988
| Autor: | E. Oosting, E. Neugebauer, J. J. Keyzer, Wilfried Lorenz |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Adult
Male Quality Control Standard sample Pathology medicine.medical_specialty Immunology 610 Medizin High-performance liquid chromatography chemistry.chemical_compound medicine Immunology and Allergy Humans Multicenter Studies as Topic ddc:610 Chromatography Plasma samples Chemistry Radioimmunoassay Middle Aged Histamine/blood Europe Human plasma Reference values Female Laboratories Quantitative analysis (chemistry) Histamine |
| Zdroj: | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. 20(4) |
| ISSN: | 0954-7894 |
| Popis: | There is an increasing interest in measuring human plasma histamine levels in various clinical conditions. A variety of 'old' and newly developed techniques are applied to meet this demand. However, the discrepancy between reported reference values for histamine in human plasma measured using this variety of techniques, suggests the existence of a certain degree of inaccuracy and imprecision. We therefore organized an external quality control study on the reliability of current histamine determinations in European laboratories. Three lyophilized plasma quality control samples, in duplicate, covering the normal and pathological range of histamine concentrations (0-45 nmol/l), two different aqueous histamine standard samples and one solvent sample were sent to 10 laboratories for the analysis of their histamine content. The following methods were used: gas chromatography-mass spectrometry (n = 2), enzymatic single isotopic assay (n = 1), fluorometric-fluoroenzymatic assay (n = 3), radioimmunoassay (n = 3) and high performance liquid chromatography (n = 2). The study was performed and evaluated according to the approved recommendations (1983) of the International Federation of Clinical Chemistry (IFCC). The target values +/- s.d. of the three plasma samples were: 39.5 +/- 4.6 nmol/l (CV = 11.6%), 2.3 +/- 2.2 nmol/l (CV = 96%) and 8.9 +/- 1.5 nmol/l (CV = 17%), respectively.(ABSTRACT TRUNCATED AT 250 WORDS) Erratum in: Clinical and experimental allergy 1991, vol. 21, p. 153 |
| Databáze: | OpenAIRE |
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