Changes in the oral ecosystem induced by the use of 8% arginine toothpaste
| Autor: | Jacob M. ten Cate, Wim Crielaard, Bernd W. Brandt, Mark J. Buijs, Michel A. Hoogenkamp, Egija Zaura, Jessica E. Koopman, Bart J. F. Keijser |
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| Přispěvatelé: | Preventieve tandheelkunde (OII, ACTA), ACTA, Preventive Dentistry, Academic Centre for Dentistry Amsterdam |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Saliva business.product_category Sucrose Arginine medicine.medical_treatment Prebiotic Biomedical Innovation Pilot Projects Biology Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Life RNA Ribosomal 16S medicine Humans Microbiome General Dentistry Mouth Toothpaste Microbiota 030206 dentistry Cell Biology General Medicine Hydrogen-Ion Concentration Healthy Volunteers stomatognathic diseases 030104 developmental biology MSB - Microbiology and Systems Biology Otorhinolaryngology chemistry Metagenomics Oral ecology Female ELSS - Earth Life and Social Sciences business Sequence Analysis Healthy Living Toothpastes |
| Zdroj: | Archives of Oral Biology, 73, 79-87 Koopman, J E, Hoogenkamp, M A, Buijs, M J, Brandt, B W, Keijser, B J F, Crielaard, W, ten Cate, J M & Zaura, E 2017, ' Changes in the oral ecosystem induced by the use of 8% arginine toothpaste ', Archives of Oral Biology, vol. 73, pp. 79-87 . https://doi.org/10.1016/j.archoralbio.2016.09.008 Archives of Oral Biology, 73, 79-87. Elsevier Archives of Oral Biology, 73, 79-87. Elsevier Limited |
| ISSN: | 0003-9969 |
| Popis: | Objective: Bacterial metabolism of arginine in the oral cavity has a pH-raising and thus, potential anti-caries effect. However, the influence of arginine on the oral microbial ecosystem remains largely unresolved. Design: In this pilot study, nine healthy individuals used toothpaste containing 8% arginine for eight weeks. Saliva was collected to determine arginolytic potential and sucrose metabolic activity at the Baseline, Week 4, Week 8 and after a two weeks Wash-out period. To follow the effects on microbial ecology, 16S rDNA sequencing on saliva and plaque samples at Baseline and Week 8 and metagenome sequencing on selected saliva samples of the same time-points was performed. Results: During the study period, the arginolytic potential of saliva increased, while the sucrose metabolism in saliva decreased. These effects were reversed during the Wash-out period. Although a few operational taxonomic units (OTUs) in plaque changed in abundance during the study period, there was no real shift in the plaque microbiome. In the saliva microbiome there was a significant compositional shift, specifically the genus Veillonella had increased significantly in abundance at Week 8.Conclusion: Indeed, the presence of arginine in toothpaste affects the arginolytic capacity of saliva and reduces its sucrose metabolic activity. Additionally, it leads to a shift in the salivary microbiome composition towards a healthy ecology from a caries point of view. Therefore, arginine can be regarded as a genuine oral prebiotic. |
| Databáze: | OpenAIRE |
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