A comparison of the Caulobacter NA1000 and K31 genomes reveals extensive genome rearrangements and differences in metabolic potential
| Autor: | Kurt T. Ash, Bert Ely, Craig Stephens, Theta Brown, LaTia Scott, Tynetta Watford |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
DNA Replication
plasmids Caulobacter Genotype genome rearrangements Immunology Replication Origin Biology Origin of replication Genome General Biochemistry Genetics and Molecular Biology Plasmid Species Specificity Caulobacter crescentus Codon lcsh:QH301-705.5 Gene Genetics Gene Rearrangement General Neuroscience Research Temperature Chromosome Gene rearrangement Chromosomes Bacterial biology.organism_classification Phenotype lcsh:Biology (General) gene insertions Genes Bacterial inversions Copper Genome Bacterial Research Article Chlorophenols |
| Zdroj: | Open Biology Open Biology, Vol 4, Iss 10 (2014) |
| ISSN: | 2046-2441 |
| Popis: | The genusCaulobacteris found in a variety of habitats and is known for its ability to thrive in low-nutrient conditions. K31 is a novelCaulobacterisolate that has the ability to tolerate copper and chlorophenols, and can grow at 4°C with a doubling time of 40 h. K31 contains a 5.5 Mb chromosome that codes for more than 5500 proteins and two large plasmids (234 and 178 kb) that code for 438 additional proteins. A comparison of the K31 and theCaulobacter crescentusNA1000 genomes revealed extensive rearrangements of gene order, suggesting that the genomes had been randomly scrambled. However, a careful analysis revealed that the distance from the origin of replication was conserved for the majority of the genes and that many of the rearrangements involved inversions that included the origin of replication. On a finer scale, numerous small indels were observed. K31 proteins involved in essential functions shared 80–95% amino acid sequence identity with theirC. crescentushomologues, while other homologue pairs tended to have lower levels of identity. In addition, the K31 chromosome contains more than 1600 genes with no homologue in NA1000. |
| Databáze: | OpenAIRE |
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