The Salt-Stress Response of the Transgenic Plum Line J8-1 and Its Interaction with the Salicylic Acid Biosynthetic Pathway from Mandelonitrile

Autor: Cesar Petri, Agustina Bernal-Vicente, José Antonio Hernández, Daniel Cantabella, Pedro Diaz-Vivancos
Přispěvatelé: Producció Vegetal, Postcollita, European Commission, Ministerio de Economía y Competitividad (España), Universidad Politécnica de Cartagena, CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
mandelonitrile
Salt Stress
01 natural sciences
lcsh:Chemistry
Mandelonitrile
chemistry.chemical_compound
salt-stress
lcsh:QH301-705.5
Chlorophyll fluorescence
Spectroscopy
chlorophyll fluorescence
Jasmonic acid
Salicylic acid
Prunus domestica
General Medicine
Plants
Genetically Modified
NPR1
Computer Science Applications
Biochemistry
Shoot
Redox signalling
Acetonitriles
salicylic acid
Thioredoxin h
Article
Catalysis
Soluble nutrients
Inorganic Chemistry
03 medical and health sciences
Biosynthesis
biochemistry
Physical and Theoretical Chemistry
Molecular Biology
J8-1 plum line
Salt-stress
Organic Chemistry
redox signalling
soluble nutrients
Biosynthetic Pathways
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
chemistry
Salts
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences
Volume 19
Issue 11
IRTA Pubpro. Open Digital Archive
Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
Digital.CSIC. Repositorio Institucional del CSIC
instname
International Journal of Molecular Sciences, Vol 19, Iss 11, p 3519 (2018)
ISSN: 1422-0067
DOI: 10.3390/ijms19113519
Popis: Salinity is considered as one of the most important abiotic challenges that affect crop productivity. Plant hormones, including salicylic acid (SA), are key factors in the defence signalling output triggered during plant responses against environmental stresses. We have previously reported in peach a new SA biosynthetic pathway from mandelonitrile (MD), the molecule at the hub of the cyanogenic glucoside turnover in Prunus sp. In this work, we have studied whether this new SA biosynthetic pathway is also present in plum and the possible role this pathway plays in plant plasticity under salinity, focusing on the transgenic plum line J8-1, which displays stress tolerance via an enhanced antioxidant capacity. The SA biosynthesis from MD in non-transgenic and J8-1 micropropagated plum shoots was studied by metabolomics. Then the response of J8-1 to salt stress in presence of MD or Phe (MD precursor) was assayed by measuring: chlorophyll content and fluorescence parameters, stress related hormones, levels of non-enzymatic antioxidants, the expression of two genes coding redox-related proteins, and the content of soluble nutrients. The results from in vitro assays suggest that the SA synthesis from the MD pathway demonstrated in peach is not clearly present in plum, at least under the tested conditions. Nevertheless, in J8-1 NaCl-stressed seedlings, an increase in SA was recorded as a result of the MD treatment, suggesting that MD could be involved in the SA biosynthesis under NaCl stress conditions in plum plants. We have also shown that the plum line J8-1 was tolerant to NaCl under greenhouse conditions, and this response was quite similar in MD-treated plants. Nevertheless, the MD treatment produced an increase in SA, jasmonic acid (JA) and reduced ascorbate (ASC) contents, as well as in the coefficient of non-photochemical quenching (qN) and the gene expression of Non-Expressor of Pathogenesis-Related 1 (NPR1) and thioredoxin H (TrxH) under salinity conditions. This response suggested a crosstalk between different signalling pathways (NPR1/Trx and SA/JA) leading to salinity tolerance in the transgenic plum line J8-1.
This work was supported by the Spanish Ministry of Economy and Competitiveness (Projects AGL2014-52563-R and INIA-RTA2013-00026-C03-00). PDV and CP thank CSIC and UPCT, respectively, as well as the Spanish Ministry of Economy and Competitiveness for their ‘Ramon and Cajal’ research contract, co-financed by FEDER funds. This work was supported by the Spanish Ministry of Economy and Competitiveness (Projects AGL2014-52563-R and INIA-RTA2013-00026-C03-00).
Databáze: OpenAIRE
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