Protein engineering of insulin: Two novel fast-acting insulins [B16Ala]insulin and [B26Ala]insulin
| Autor: | Shi-Yin Yao, Shangquan Zhu, You-Min Feng, Zhou Zhang, Yue-Hua Tang |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Insulin medicine.medical_treatment Biological activity Biology biology.organism_classification General Biochemistry Genetics and Molecular Biology Yeast Pichia pastoris Endocrinology In vivo Internal medicine Insulin receptor substrate Gene expression medicine Potency General Agricultural and Biological Sciences General Environmental Science |
| Zdroj: | Science in China Series C. 46:474 |
| ISSN: | 1006-9305 |
| DOI: | 10.1360/01yc0295 |
| Popis: | Blood glucose lowering assay proved that [B16Ala]insulin and [B26Ala]insulin exhibit potency of acute blood glucose lowering in normal pigs, which demonstrates that they are fast-acting insulin. Single-chain precursor of [B16Ala]insulin and [B26Ala]insulin is [B16Ala]PIP and [B26Ala]PIP, respectively, which are suitable for gene expression. Secretory expression level of the precursors in methylotrophic yeast Pichia pastoris was quite high, 650 mg/L and 130 mg/L, respectively. In vivo biological assay showed that the two fast-acting insulins have full or nearly full biological activity. So both [B16Ala]insulin and [B26Ala]insulin can be well developed as fast-acting insulin for clinic use. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink