Herpes simplex virus DNA synthesis in a partially purified soluble extract from infected cells
| Autor: | P F Pignatti, U Bertazzoni, E Cassai |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
DNA polymerase
viruses DNA polymerase II Deoxyribonucleotides Immunology DNA-Directed DNA Polymerase Microbiology DNA polymerase delta Cell Line Single-stranded binding protein Replication factor C Virology Herpes virus Simplexvirus DNA synthesis biology DNA replication Molecular biology Insect Science DNA Viral biology.protein Replicon Primase DNA polymerase I Research Article |
| Zdroj: | Journal of Virology. 32:1033-1036 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | HEp-2 cells were infected with herpes simplex virus type 1 and extracted with 0.25% Triton X-100 and 0.1 M NaCl. The extract was sedimented on sucrose gradients, and the fractions containing the endogenous DNA polymerizing activity (replication complex) were collected. The properties of the replication complex were partially characterized. Under optimal conditions 375 pmol of dTMP per micrograms of DNA was incorporated, which corresponds to about 50% replication of preexisting viral DNA. The replication complex was shown to contain only DNA of viral origin by its density in CsCl. By using specific assays for DNA polymerases alpha, beta, gamma, and herpes simplex virus, we found that only the viral DNA polymerase copurified with the replication complex. |
| Databáze: | OpenAIRE |
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