Functional and biophysical analyses of the class XIV Toxoplasma gondii Myosin D

Autor: Miklós Nyitrai, Stefan Weiss, L. David Sibley, Stanislas Tomavo, Rolf Stratmann, Dominique Soldati, Frédéric Delbac, Angelika Herm-Götz, Michael A. Geeves
Přispěvatelé: Hygieneinstitut, Universität Heidelberg [Heidelberg], Zentrum für Molekular Biologie, Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Centre National de la Recherche Scientifique (CNRS), Departement of Biosciences, University of Kent [Canterbury], Department of Biophysics, University of Pecs, Heidelberg Institut für Planzenwissenschaften, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), departement of Molecular Microbiology, Washington University School of Medicine, Department of Microbiology and Molecular Medicine, University of Geneva [Switzerland]-Faculty of Medicine, Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Centre National de la Recherche Scientifique (CNRS)-Université d'Auvergne - Clermont-Ferrand I (UdA), university of Pécs, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Universität Heidelberg [Heidelberg] = Heidelberg University, Laboratorium für Molekulare Biologie - Genzentrum-Institut für Biochemie der LMU München, Ludwig-Maximilians-Universität München (LMU), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Department of Molecular Microbiology, University of Washington [Seattle], Department of Biosciences, Université de Genève = University of Geneva (UNIGE)-Faculty of Medicine
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Zdroj: Journal of muscle research and cell motility.
Journal of muscle research and cell motility., 2006, 27(2), pp.139-51
Journal of Muscle Research and Cell Motility
Journal of Muscle Research and Cell Motility, 2006, 27 (2), pp.139-51. ⟨10.1007/s10974-005-9046-1⟩
Journal of Muscle Research & Cell Motility
Journal of Muscle Research & Cell Motility, 2006, 27 (2), pp.139-51. ⟨10.1007/s10974-005-9046-1⟩
Journal of Muscle Research and Cell Motility, Springer Verlag, 2006, 27 (2), pp.139-51. ⟨10.1007/s10974-005-9046-1⟩
Journal of Muscle Research and Cell Motility, Vol. 27, No 2 (2006) pp. 139-151
ISSN: 0142-4319
1573-2657
DOI: 10.1007/s10974-005-9046-1⟩
Popis: International audience; The obligate intracellular parasite Toxoplasma gondii uses gliding motility to migrate across the biological barriers of the host and to invade cells. This unique form of locomotion requires an intact actin cytoskeleton and involves at least one motor protein (TgMyoA) that belongs to the class XIV of the myosin superfamily. TgMyoA is anchored in the inner membrane complex and is essential for the gliding motion, host cell invasion and egress of T. gondii tachyzoites. TgMyoD is the smallest T. gondii myosin and is structurally very closely related to TgMyoA. We show here that TgMyoD exhibits similar transient kinetic properties as the fast single-headed TgMyoA. To determine if TgMyoD also contributes to parasite gliding motility, the TgMyoD gene was disrupted by double homologous recombination. In contrast to TgMyoA, TgMyoD gene is dispensable for tachyzoite propagation and motility. Parasites lacking TgMyoD glide normally and their virulence is not compromised in mice. The fact that TgMyoD is predominantly expressed in bradyzoites explains the absence of a phenotype observed with myodko in tachyzoites and does not exclude a role of this motor in gliding that would be restricted to the cyst forming but nevertheless motile stage of the parasite.
Databáze: OpenAIRE
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