Galectin-3, a novel centrosome-associated protein, required for epithelial morphogenesis
| Autor: | Delphine Delacour, Annett Koch, Ralf Jacob, Françoise Poirier |
|---|---|
| Přispěvatelé: | Department of cell biology and cell pathology, University of Marburg, Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
animal structures
Centriole Galectin 3 Morphogenesis [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology MESH: Centrosome Epithelium MESH: Dogs 03 medical and health sciences Mice 0302 clinical medicine Dogs MESH: Cilia MESH: RNA Small Interfering medicine otorhinolaryngologic diseases Basal body Animals MESH: Animals MESH: Gene Silencing Cilia Gene Silencing RNA Small Interfering Molecular Biology MESH: Mice Tissue homeostasis 030304 developmental biology Epithelial polarity Centrioles Centrosome 0303 health sciences MESH: Centrioles Cilium Epithelial Cells Cell Biology Articles MESH: Morphogenesis MESH: Galectin 3 Cell biology stomatognathic diseases MESH: Epithelium medicine.anatomical_structure MESH: Epithelial Cells 030220 oncology & carcinogenesis |
| Zdroj: | Molecular Biology of the Cell Molecular Biology of the Cell, American Society for Cell Biology, 2010, 21 (2), pp.219-31. ⟨10.1091/mbc.E09-03-0193⟩ |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.E09-03-0193⟩ |
| Popis: | We investigated the role of galectin-3 on polarization of epithelial renal cells, using three-dimensional cultures of MDCK cells and also galectin-3 null mutant mouse kidneys. Collectively, data show that the absence of galectin-3 influences the stabilization of centrosomes and primary cilia, with effects on epithelial cell organization. Galectin-3 is a β-galactoside–binding protein widely expressed in all epithelia where it is involved in tissue homeostasis and cancer progression. We recently reported unique abnormalities in the identity of membrane domains in galectin-3 null mutant mice, suggesting that galectin-3 may participate in epithelial polarity program. We investigated the potential role of galectin-3 on early events in polarization of epithelial renal cells, using three-dimensional cultures of MDCK cells and also galectin-3 null mutant mouse kidneys. We show that depletion in galectin-3 systematically leads to severe perturbations of microtubular network associated with defects in membrane compartimentation, both in vitro and in vivo. Moreover, the absence of galectin-3 impinges on the morphology of the primary cilium, which is three times longer and unusually shaped. By immunological and biochemical approaches, we could demonstrate that endogenous galectin-3 is normally associated with basal bodies and centrosomes, where it closely interacts with core proteins, such as centrin-2. However, this association transiently occurs during the process of epithelial polarization. Interestingly, galectin-3–depleted cells contain numerous centrosome-like structures, demonstrating an unexpected function of this protein in the formation and/or stability of the centrosomes. Collectively, these data establish galectin-3 as a key determinant in epithelial morphogenesis via its effect on centrosome biology. |
| Databáze: | OpenAIRE |
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