A kinase-encoding gene from Colletotrichum trifolii complements a colonial growth mutant of Neurospora crassa
| Autor: | S. Oved, C. Huang, Oded Yarden, G. M. Truesdell, T. L. Buhr, Martin B. Dickman |
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| Rok vydání: | 1996 |
| Předmět: |
Colletotrichum trifolii
Mutant Genes Fungal Molecular Sequence Data Gene Dosage Germ tube Protein Serine-Threonine Kinases Neurospora crassa Fungal Proteins Complementary DNA Gene Expression Regulation Fungal Sequence Homology Nucleic Acid Genetics Amino Acid Sequence RNA Messenger Cloning Molecular Molecular Biology Gene Fungal protein biology Base Sequence Sequence Homology Amino Acid fungi Genetic Complementation Test Fungal genetics RNA Fungal Sequence Analysis DNA biology.organism_classification Biochemistry Mutation Mitosporic Fungi |
| Zdroj: | Moleculargeneral genetics : MGG. 251(5) |
| ISSN: | 0026-8925 |
| Popis: | Colletotrichum trifolii is a fungal pathogen which is responsible for anthracnose disease of alfalfa. To initiate research on molecular communication in this fungus, a kinase-encoding gene (TB3) and the corresponding cDNA were cloned and sequenced. The deduced amino acid sequence of TB3 closely resembles that of a Neurospora crassa serine/threonine protein kinase, COT1, required for hyphal elongation and branching. The C-terminal catalytic domains of TB3 and COT1 are highly conserved but the N-terminal regions are divergent, particularly in the homopolymeric glutamine repeats of TB3. Northern analysis indicated that TB3 expression was highest 1 h after inducing conidial germination and 1 h before germ tubes were first observed. Expression of TB3 transcripts returned to constitutive levels by 4 h after induction of germination. TB3 complemented the cot-I mutant of Neurospora crassa, demonstrating the functional conservation of this kinase between a pathogenic and a saprophytic fungus. |
| Databáze: | OpenAIRE |
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