Probiotic attributes, antioxidant, anti-inflammatory and neuromodulatory effects of Enterococcus faecium CFR 3003: in vitro and in vivo evidence
| Autor: | Muralidhara, Gokul Krishna, Siddalingaiya Gurudutt Prapulla, G. Divyashri |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Microbiology (medical) Time Factors Antioxidant medicine.medical_treatment Enterococcus faecium Anxiety medicine.disease_cause Microbiology Neuroprotection Antioxidants Cell Line law.invention Proinflammatory cytokine Protein Carbonylation Mice Random Allocation Probiotic Lactobacillus rhamnosus law In vivo medicine Animals Trypsin Cecum Behavior Animal biology Lacticaseibacillus rhamnosus Tumor Necrosis Factor-alpha Macrophages Probiotics General Medicine Hydrogen-Ion Concentration biology.organism_classification Pepsin A Anti-Bacterial Agents Interleukin-10 Oxidative Stress Oxidative stress |
| Zdroj: | Journal of Medical Microbiology. 64:1527-1540 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/jmm.0.000184 |
| Popis: | Accumulating evidence suggests that probiotic bacteria play a vital role in modulating various aspects integral to the health and well-being of humans. In the present study, probiotic attributes and the antioxidant, anti-inflammatory and neuromodulatory potential of Enterococcus faecium CFR 3003 were investigated by employing suitable model systems. E. faecium exhibited robust resistance to gastrointestinal stress conditions as it could withstand acid stress at pH 1.5, 2 and 3. The bacterium also survived at a bile salt concentration of 0.45 %, and better tolerance was observed towards pepsin and trypsin. E. faecium produced lactic acid as a major metabolic product, followed by butyric acid. Lyophilized cell-free supernatant (LCS) of E. faecium exhibited significant antioxidant capacity evaluated against 1,1-diphenyl-2-picryl-hydrazyl, ascorbate auto-oxidation, oxygen radical absorbance and reducing power. Interestingly, E. faecium, Lactobacillus rhamnosus GG MTCC 1408 and LCS showed a significant anti-inflammatory effect by negatively modulating TNF-α production and upregulating IL-10 levels in LPS-stimulated macrophage cell lines. In an in vivo mice model, the propensity of probiotic supplements to modulate endogenous oxidative markers and redox status in brain regions was assessed. Young mice provided with oral supplements (daily for 28 days) of E. faecium and L. rhamnosus exhibited diminished oxidative markers in the brain and enhanced activities of antioxidant enzymes with a concomitant increase in γ-aminobutyric acid and dopamine levels. Collectively, our findings clearly suggest the propensity of these bacteria to protect against tissue damage mediated through free radicals and inflammatory cytokines. Although the underlying molecular mechanisms need further studies, it is tempting to speculate that probiotics confer a neuroprotective advantage in vivo against oxidative damage-mediated neurodegenerative conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|