Determination of narciclasine in serum by reversed-phase high-performance liquid chromatography: comparison of amperometric, ultraviolet photometric and fluorescence detection
| Autor: | Karel Štulík, Věra Pacáková, Francesco M. Veronese, Paolo Caliceti, Ivana Švagrová |
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| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Detection limit
Aqueous solution Chromatography Chemistry Narciclasine Analytical chemistry Antineoplastic Agents Mice Inbred Strains General Chemistry Reversed-phase chromatography Fluorescence High-performance liquid chromatography Amperometry Fluorescence spectroscopy Phenanthridines Mice Alkaloids Spectrometry Fluorescence Amaryllidaceae Alkaloids Electrochemistry Animals Indicators and Reagents Spectrophotometry Ultraviolet Chromatography High Pressure Liquid |
| Popis: | Narciclasine was determined in the blood of mice by reversed-phase high-performance liquid chromatography, using a C18 stationary phase and a mobile phase of methanol—0.025 M potassium dihydrogen phosphate (50:50, v/v) of pH 5.5. Amperometric detection at a carbon fibre array working electrode held at + 1.8 V (Ag/AgCl) permitted determination down to concentrations of 10 and 15.4 ng ml−1 (at a signal-to-noise ratio of 2) in aqueous solution and in serum, respectively. Fluorescence detection (excitation and emission wavelengths of 360 and 480 nm, respectively) exhibited somewhat poorer sensitivities for aqueous and serum samples: the respective limits of detection were 25 and 32 ng ml−1 at a signal-to-noise ratio of 2. Both the amperometric and the fluorescence detection were free from interference from blood components, but the fluorescence measurement required a post-column pH adjustment. UV photometric detection at 254 nm exhibited detection limits of 15 and 65 ng ml−1 in aqueous samples and in serum, respectively, and suffered from interferences from blood components that strongly absorbed in the ultraviolet region. All three detection techniques exhibited good linearity and precision. |
| Databáze: | OpenAIRE |
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