Dissecting the Roles of Cuticular Wax in Plant Resistance to Shoot Dehydration and Low-Temperature Stress in Arabidopsis
| Autor: | Prakash Venglat, Raju Soolanayakanahally, Tawhidur Rahman, Mingxuan Shao, Abidur Rahman, Xiao Qiu, Karen K. Tanino, Shankar Pahari |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Acclimatization Arabidopsis 01 natural sciences lcsh:Chemistry Spectroscopy Fourier Transform Infrared Arabidopsis thaliana lcsh:QH301-705.5 Spectroscopy chemistry.chemical_classification Wax biology Chemistry General Medicine Adaptation Physiological Lipids Computer Science Applications Cold Temperature cuticular wax Plant cuticle visual_art Shoot visual_art.visual_art_medium Hydrophobic and Hydrophilic Interactions Plant Shoots alkane Plant Development low temperature freezing Gas Chromatography-Mass Spectrometry Article Catalysis Inorganic Chemistry 03 medical and health sciences Stress Physiological Botany medicine Cold acclimation biochemistry Dehydration Physical and Theoretical Chemistry Molecular Biology Alleles Alkane Arabidopsis Proteins Organic Chemistry fungi dehydration Lipid Metabolism biology.organism_classification medicine.disease 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Waxes Mutation 010606 plant biology & botany stress avoidance |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 4 International Journal of Molecular Sciences, Vol 22, Iss 1554, p 1554 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22041554 |
| Popis: | Cuticular waxes are a mixture of hydrophobic very-long-chain fatty acids and their derivatives accumulated in the plant cuticle. Most studies define the role of cuticular wax largely based on reducing nonstomatal water loss. The present study investigated the role of cuticular wax in reducing both low-temperature and dehydration stress in plants using Arabidopsis thaliana mutants and transgenic genotypes altered in the formation of cuticular wax. cer3-6, a known Arabidopsis wax-deficient mutant (with distinct reduction in aldehydes, n-alkanes, secondary n-alcohols, and ketones compared to wild type (WT)), was most sensitive to water loss, while dewax, a known wax overproducer (greater alkanes and ketones compared to WT), was more resistant to dehydration compared to WT. Furthermore, cold-acclimated cer3-6 froze at warmer temperatures, while cold-acclimated dewax displayed freezing exotherms at colder temperatures compared to WT. Gas Chromatography-Mass Spectroscopy (GC-MS) analysis identified a characteristic decrease in the accumulation of certain waxes (e.g., alkanes, alcohols) in Arabidopsis cuticles under cold acclimation, which was additionally reduced in cer3-6. Conversely, the dewax mutant showed a greater ability to accumulate waxes under cold acclimation. Fourier Transform Infrared Spectroscopy (FTIR) also supported observations in cuticular wax deposition under cold acclimation. Our data indicate cuticular alkane waxes along with alcohols and fatty acids can facilitate avoidance of both ice formation and leaf water loss under dehydration stress and are promising genetic targets of interest. |
| Databáze: | OpenAIRE |
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