Expression of bclxs and c-myc in atretic follicles of mouse ovary
| Autor: | Shalmali J Dharma, Tarala D. Nandedkar |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
endocrine system
medicine.medical_specialty TUNEL assay Follicular atresia Obstetrics and Gynecology Ovary Biology Andrology medicine.anatomical_structure Pregnant mare serum Endocrinology Reproductive Medicine Apoptosis Internal medicine medicine Immunohistochemistry Mouse Ovary Gene Developmental Biology |
| Zdroj: | Reproductive BioMedicine Online. 3:221-225 |
| ISSN: | 1472-6483 |
| DOI: | 10.1016/s1472-6483(10)62040-8 |
| Popis: | At birth, in the human female, one million follicles are present, out of which only 450 ovulate during the reproductive lifespan while the remainder of the 99.9% follicles degenerate. It is therefore important to understand the regulation of follicular atresia. Immature mice injected with pregnant mare serum gonadotrophin (PMSG) were used as a model for follicular atresia. In this model, we demonstrated by various methods, including the TdT-mediated dUTP nick end labelling (TUNEL) technique, that granulosa cells in atretic follicles undergo apoptosis. In eukaryotic cells, apoptosis is regulated by genes such as bcl(2) and c-myc. Regulation of apoptosis in the ovary by these proteins/genes was studied using the mouse model. bcl(2) is anti-apoptotic; however, bcl(xs), a member of the bcl(2) family, is apoptotic. Immunohistochemical localization of bcl(xs) in the granulosa cells of atretic follicles suggested its role in follicular atresia. Expression of c-myc was studied by in-situ hybridization in the mouse follicular atresia model. In the atretic follicles, expression of c-myc in granulosa cells was observed. Thus our studies indicate that bcl(xs) regulates granulosa cell apoptosis, while c-myc may also play a role in cell death during follicular atresia. |
| Databáze: | OpenAIRE |
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