Deep transcriptomic profiling of M1 macrophages lacking Trpc3
| Autor: | Lutz Birnbaumer, Sumeet Solanki, Oluwatomisin T. Atolagbe, Sivarajan Kumarasamy, Bina Joe, Guillermo Vazquez |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
CIENCIAS MÉDICAS Y DE LA SALUD Inmunología Down-Regulation BIOLOGIA CELULAR Bone Marrow Cells 030204 cardiovascular system & hematology Biology TRPC3 Article LIPIDOS Transcriptome Mice 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement Ca2+/calmodulin-dependent protein kinase Animals KEGG MARCADORES BIOLOGICOS Cells Cultured Chemokine CCL2 TRPC Cation Channels Mice Knockout M1 Macrophages Multidisciplinary Cell adhesion molecule Gene Expression Profiling Macrophages purl.org/becyt/ford/3.1 [https] Lipid signaling Netrin-1 SISTEMA INMUNOLOGICO Molecular biology Up-Regulation 3. Good health Cell biology Medicina Básica 030104 developmental biology Unfolded protein response RNA purl.org/becyt/ford/3 [https] |
| Zdroj: | Scientific Reports. 2017;7:39867 Repositorio Institucional (UCA) Pontificia Universidad Católica Argentina instacron:UCA Scientific Reports CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| Popis: | Fil: Kumarasamy, Sivarajan. University of Toledo. College of Medicine and Life Sciences. Department of Physiology and Pharmacology. Center for Hypertension and Personalized Medicine; Estados Unidos Fil: Solanki, Sumeet. University of Toledo. College of Medicine and Life Sciences. Department of Physiology and Pharmacology. Center for Hypertension and Personalized Medicine; Estados Unidos Fil: Atolagbe, Oluwatomisin T. University of Toledo. College of Medicine and Life Sciences. Department of Physiology and Pharmacology. Center for Hypertension and Personalized Medicine; Estados Unidos Fil: Joe, Bina. University of Toledo. College of Medicine and Life Sciences. Department of Physiology and Pharmacology. Center for Hypertension and Personalized Medicine; Estados Unidos Fil: Birnbaumer, Lutz. National Institute of Environmental Health Sciences. Laboratory of Neurobiology; Estados Unidos Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina Fil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vazquez, Guillermo. University of Toledo. College of Medicine and Life Sciences. Department of Physiology and Pharmacology. Center for Hypertension and Personalized Medicine; Estados Unidos Abstract: In previous studies using mice with macrophage-specific loss of TRPC3 we found a significant, selective effect of TRPC3 on the biology of M1, or inflammatory macrophages. Whereas activation of some components of the unfolded protein response and the pro-apoptotic mediators CamkII and Stat1 was impaired in Trpc3-deficient M1 cells, gathering insight about other molecular signatures within macrophages that might be affected by Trpc3 expression requires an alternative approach. In the present study we conducted RNA-seq analysis to interrogate the transcriptome of M1 macrophages derived from mice with macrophage-specific loss of TRPC3 and their littermate controls. We identified 160 significantly differentially expressed genes between the two groups, of which 62 were upregulated and 98 downregulated in control vs. Trpc3-deficient M1 macrophages. Gene ontology analysis revealed enrichment in processes associated to cellular movement and lipid signaling, whereas the enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways included networks for calcium signaling and cell adhesion molecules, among others. This is the first deep transcriptomic analysis of macrophages in the context of Trpc3 deficiency and the data presented constitutes a unique resource to further explore functions of TRPC3 in macrophage biology. |
| Databáze: | OpenAIRE |
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