Molecular characterization of maize bHLH transcription factor (ZmKS), a new ZmOST1 kinase substrate
| Autor: | Dolors Ludevid, Montserrat Pagès, Agnese Rabissi, Victoria Lumbreras, Belmiro Vilela, Francisco A. Culiáñez-Macià |
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| Přispěvatelé: | Ministerio de Educación y Ciencia (España), CSIC-IRTA-UAB-UB - Centre de Recerca Agrigenómica (CRAG) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Molecular Sequence Data Arabidopsis Gene Expression E-box Plant Science 01 natural sciences Zea mays 03 medical and health sciences Bimolecular fluorescence complementation Genetics Basic Helix-Loop-Helix Transcription Factors Amino Acid Sequence Protein kinase A Transcription factor Plant Proteins biology Kinase Activator (genetics) fungi Stomatal activity food and beverages General Medicine Abiotic stress biology.organism_classification Plants Genetically Modified Molecular biology Cell biology bHLH transcription factor 030104 developmental biology ABA-repressed Phosphorylation Agronomy and Crop Science 010606 plant biology & botany Abscisic Acid |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Popis: | In order to identify potential substrates of the maize kinase in the ABA signalling network, ZmOST1 was used as bait against a library of cDNAs from dehydrated young leaves. A ZmOST1-interactive polypeptide ZmKS (gene locus tag: GRMZM2G114873), showing homology with the Arabidopsis thaliana basic helix-loop-helix (bHLH) DNA-binding transcription factor was identified. Using a comparative genomic approach, the ZmKS corresponding protein was identified as conceptual translated bHLH transcription factor ABA-responsive kinase substrate. ZmKS is localized in the nucleus, shows a potential binding specificity preferentially detectable on cis-acting E-box like heptameric motifs CCACTTG and CAAGTTG, and is phosphorylated by maize protein kinase ZmOST1. ZmKS is expressed in embryo, leaf and root, expression being affected by ABA and osmotic stress. Transgenic Arabidopsis plants, with gain of ZmKS function, show a delay in germination and a transcriptional stomatal opening-facilitator activity, switchover upon ZmKS phosphorylation, suggesting that ZmKS is an ABA-repressed trans-acting activator. Research in M.P laboratory was supported grant from the Spanish Ministry of Science and Education BIO2012-3186 Centre de Recerca en Agrigenòmica 20 (CRAG), Consortium CSIC-IRTA-UAB-UB. Campus UAB − Edifici CRAG, 21 Cerdanyola del Vallès 08193 Barcelona (Spain), Tel: +34 935636600, Fax: 22 +34935636601 |
| Databáze: | OpenAIRE |
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