Preventing biofilm formation and associated occlusion by biomimetic glycocalyxlike polymer in central venous catheters
| Autor: | Christophe Beloin, Aude Bernardin, Marc Esteve, Jean-Marc Ghigo, Vincent Semetey, Windy Mussard, Irène Kriegel, Ashwini Chauhan |
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| Přispěvatelé: | Génétique des Biofilms, Institut Pasteur [Paris], Institut Curie [Paris], Institut Curie-Hôpital, Physico-Chimie-Curie (PCC), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC), This work was supported by the Agence Nationale de la Recherche (ANR) (grants ANR-07-EMPB-004-01 and ANR-08-PCVI-0012), the Institut Curie/Institut Pasteur (Programme Incitatif Coopératif (PIC)/Programme Transversal de Recherche (PTR) maladies nosocomiales), and the French government's Investissement d'Avenir program, Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant ANR-10-LABX-62-IBEID)., ANR-07-EMPB-0004,BIOSILICONE,Traitement de surface de silicone à l'aide de copolymères(2007), ANR-08-PCVI-0012,3DPHOTOPOL,Photopolymérisation de microstructures 3D pour le contrôle de l'architecture et des communications multicellulaires(2008), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Institut Pasteur [Paris] (IP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2014 |
| Předmět: |
Male
MESH: Biomimetic Materials/therapeutic use medicine.medical_treatment medicine.disease_cause Bacterial Adhesion Biomimetic Materials Occlusion Immunology and Allergy MESH: Staphylococcal Infections/prevention & control Central Venous Catheters MESH: Animals 0303 health sciences Staphylococcal Infections Thrombosis 3. Good health Infectious Diseases Staphylococcus aureus Central venous catheter Modern medicine MESH: Rats MESH: Biofilms/growth & development anti-adhesion Methylcellulose MESH: Central Venous Catheters/adverse effects Glycocalyx Article Microbiology medical devices 03 medical and health sciences In vivo MESH: Central Venous Catheters/microbiology MESH: Pseudomonas Infections/prevention & control medicine Animals Pseudomonas Infections MESH: Bacterial Adhesion 030304 developmental biology MESH: Catheter-Related Infections/prevention & control 030306 microbiology Pseudomonas aeruginosa business.industry MESH: Glycocalyx/microbiology Biofilm medicine.disease [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Male Rats MESH: Methylcellulose/analogs & derivatives Biofilms Catheter-Related Infections business |
| Zdroj: | Journal of Infectious Diseases Journal of Infectious Diseases, Oxford University Press (OUP), 2014, 210 (9), pp.1347-1356. ⟨10.1093/infdis/jiu249⟩ Journal of Infectious Diseases, 2014, 210 (9), pp.1347-1356. ⟨10.1093/infdis/jiu249⟩ |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/jiu249⟩ |
| Popis: | International audience; The use of catheters and other implanted devices is constantly increasing in modern medicine. Although catheters improve patients' healthcare, the hydrophobic nature of their surface material promotes protein adsorption and cell adhesion. Catheters are therefore prone to complications, such as colonization by bacterial and fungal biofilms, associated infections, and thrombosis. Here we describe the in vivo efficacy of biologically inspired glycocalyxlike antiadhesive coatings to inhibit Staphylococcus aureus and Pseudomonas aeruginosa colonization on commercial totally implantable venous access ports (TIVAPs) in a clinically relevant rat model of biofilm infection. Although noncoated TIVAPs implanted in rats were heavily colonized by the 2 biofilm-forming pathogens with a high percentage of occlusion, coating TIVAPs reduced their initial adherence and subsequently led to 4-log reduction in biofilm formation and reduced occlusion. Our antiadhesive approach is a simple and generalizable strategy that could be used to minimize clinical complications associated with the use of implantable medical devices. |
| Databáze: | OpenAIRE |
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