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It has been estimated that spermatozoa can last from 200-32,000 years (Stoss & Donaldson, 1983; Suquet et al., 2000). According to Kopeika et al. (2007), several methods of fish sperm storage has been practised including stored in medium with saturated gases, preservation at temperature above zero, in a frozen state as well as by drying. However, the low temperature approaches have been successful in fish sperm cryopreservation. Thus, cryopreservation technology offers the best means for long term storage of fish semen. |
