Clinical parameters of inflammatory bowel disease in children do not correlate with four common polymorphisms of the transforming growth factor β1 gene
| Autor: | Grzegorz Węgrzyn, Piotr M. Wierzbicki, Zbigniew Kmieć, Anna Liberek, Katarzyna Plata-Nazar, Tomasz Liberek, Grazyna Łuczak, Joanna Jakóbkiewicz-Banecka, Barbara Kamińska, Joanna Świderska, Grazyna Sikorska-Wiśniewska, Krzysztof Marek, Anna Kloska |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Adolescent Genotype medicine.medical_treatment Population Inflammatory bowel disease General Biochemistry Genetics and Molecular Biology Transforming Growth Factor beta1 Intestinal mucosa Crohn Disease Gene Frequency Medicine Humans Genetic Testing RNA Messenger Intestinal Mucosa education Child Crohn's disease education.field_of_study Polymorphism Genetic business.industry Reverse Transcriptase Polymerase Chain Reaction Infant medicine.disease Inflammatory Bowel Diseases Ulcerative colitis Immunohistochemistry digestive system diseases Cytokine Case-Control Studies Child Preschool Immunology Colitis Ulcerative Female Gene polymorphism business Transforming growth factor |
| Zdroj: | Scopus-Elsevier Europe PubMed Central Knowledge Base of the University of Gdańsk |
| ISSN: | 1734-154X |
| Popis: | Transforming growth factor β1 (TGF-β1) is a cytokine affecting cell proliferation and development, which also has an immunomodulatory activity. Correlations between polymorphisms of the TGF-β1 gene and clinical parameters of inflammatory bowel disease (IBD) were reported previously in adults. Here, we tested whether such correlations occur in pediatric patients suffering from IBD. One hundred and four pediatric IBD patients were involved in this study. Among them, 36 were diagnosed with Crohn's Disease (CD) and 68 were diagnosed with ulcerative colitis (UC). The control group consisted of 103 children, in which IBD was excluded. TGF-β1 levels were determined in plasma and intestinal mucosa samples. The presence of the TGF β1 protein and the amount of TGF β1 mRNA were estimated in intestinal mucosa by immunohistochemistry and reverse transcription Real-Time PCR, respectively. Four common polymorphisms of the TGF-β1 gene were investigated: -800G/A, -509C/T, 869T/C and 915G/C. No significant correlation between TGF-β1 genotypes and (i) TGF-β1 levels in plasma and tissue samples, (ii) TGF-β1 gene expression efficiency in intestinal mucosa, (iii) IBD clinical parameters and (iv) inflammatory activity could be detected in children suffering from IBD. We conclude that, contrary to previous suggestions, the four common polymorphisms of the TGF-β1 gene do not influence the susceptibility to or clinical parameters of IBD in the tested population of children. |
| Databáze: | OpenAIRE |
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