| Autor: |
Juliana Martins Machado, Isabela Amorim Gonçalves Pereira, Ana Clara Gontijo Maia, Mariana Ferraz Chaves Francisco, Lais Moreira Nogueira, Isadora Braga Gandra, Anna Julia Ribeiro, Kamila Alves Silva, Carlos Ananias Aparecido Resende, Jonatas Oliveira da Silva, Michelli dos Santos, Ana Alice Maia Gonçalves, Grasiele de Sousa Vieira Tavares, Miguel Angel Chávez-Fumagalli, Mariana Campos da Paz da Paz, Rodolfo Cordeiro Giunchetti, Manoel Otávio C. Rocha, Ana Thereza Chaves, Eduardo Antônio Ferraz Coelho, Alexsandro S. Galdino |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Popis: |
Background: Chagas disease is still a neglected disease considered a public health problem. Early diagnosis of cases is important to improve the prognosis of infected patients and prevent transmission. Serological tests are the method of choice for diagnosis. However, two serological tests are currently recommended to confirm positive cases. In this sense, more sensitive and specific serological tests need to be developed to overcome the problems currently faced. This study aimed to develop a new recombinant multiepitope protein for the diagnosis of Chagas disease, hereafter named rTC. Methods: The rTC was constructed based on amino acid sequences from different combinations of Trypanossoma cruzi antigens in the same polypeptide and tested in ELISA for detecting different Chagas disease. Results: rTC1 was able to discriminate between indeterminate (IND) and cardiac (CARD) cases and cross-reactive diseases, and healthy samples, with 96.6% sensitivity and 97.96% specificity, respectively. Conclusion: These data suggest a preliminary study that rTC1 has the po-tential to be tested in future studies against a larger serological panel for the diagnosis of Chagas disease. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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