Grapefruit seed extract effectively inhibits the Candida albicans biofilms development on polymethyl methacrylate denture-base resin

Autor: Yuki Arai, Reiko Tokuyama-Toda, Noriyuki Wakabayashi, Yusuke Takebe, Seiko Tatehara, Chiaki Tsutsumi-Arai, Shinji Ide, Kazuhito Satomura, Kensuke Takakusaki, Chika Terada-Ito, Takahiro Imamura
Rok vydání: 2019
Předmět:
Cell Membranes
Yeast and Fungal Models
Grapefruit seed extract
Pathology and Laboratory Medicine
Fluorescence Microscopy
0302 clinical medicine
Candida albicans
Medicine and Health Sciences
Electron Microscopy
Acrylic resin
Candida
Fungal Pathogens
Colony-forming unit
Microscopy
0303 health sciences
Multidisciplinary
biology
Chemistry
Eukaryota
Light Microscopy
Corpus albicans
Resins
Synthetic
Experimental Organism Systems
Distilled water
Medical Microbiology
visual_art
Seeds
visual_art.visual_art_medium
Saccharomyces Cerevisiae
Medicine
Scanning Electron Microscopy
Pathogens
Cellular Structures and Organelles
Citrus paradisi
Research Article
Science
Mycology
Research and Analysis Methods
Microbiology
Saccharomyces
03 medical and health sciences
Model Organisms
Humans
Polymethyl Methacrylate
Microbial Pathogens
030304 developmental biology
Plant Extracts
Organisms
Fungi
Biofilm
Biology and Life Sciences
Cell Biology
030206 dentistry
biology.organism_classification
Yeast
Specimen Preparation and Treatment
Biofilms
Animal Studies
Nuclear chemistry
Zdroj: PLoS ONE, Vol 14, Iss 5, p e0217496 (2019)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0217496
Popis: This study aimed to investigate the cleansing effects of grapefruit seed extract (GSE) on biofilms of Candida albicans (C. albicans) formed on denture-base resin and the influence of GSE on the mechanical and surface characteristics of the resin. GSE solution diluted with distilled water to 0.1% (0.1% GSE) and 1% (1% GSE) and solutions with Polident® denture cleansing tablet dissolved in distilled water (Polident) or in 0.1% GSE solution (0.1% G+P) were prepared as cleansing solutions. Discs of acrylic resin were prepared, and the biofilm of C. albicans was formed on the discs. The discs with the biofilm were treated with each solution for 5 min at 25°C. After the treatment, the biofilm on the discs was analyzed using a colony forming unit (CFU) assay, fluorescence microscopy, and scanning electron microscopy (SEM). In order to assess the persistent cleansing effect, the discs treated with each solution for 5 min were aerobically incubated in Yeast Nitrogen Base medium for another 24 h. After incubation, the persistent effect was assessed by CFU assay. Some specimens of acrylic resin were immersed in each solution for 7 days, and changes in surface roughness (Ra), Vickers hardness (VH), flexural strength (FS), and flexural modulus (FM) were evaluated. As a result, the treatment with 1% GSE for 5 min almost completely eliminated the biofilm formed on the resin; whereas, the treatment with 0.1% GSE, Polident, and 0.1% G+P for 5 min showed a statistically significant inhibitory effect on biofilms. In addition, 0.1% GSE and 0.1% G+P exerted a persistent inhibitory effect on biofilms. Fluorescence microscopy indicated that Polident mainly induced the death of yeast, while the cleansing solutions containing at least 0.1% GSE induced the death of hyphae as well as yeast. SEM also revealed that Polident caused wrinkles, shrinkage, and some deep craters predominantly on the cell surfaces of yeast, while the solutions containing at least 0.1% GSE induced wrinkles, shrinkage, and some damage on cell surfaces of not only yeasts but also hyphae. No significant changes in Ra, VH, FS, or FM were observed after immersion in any of the solutions. Taken together, GSE solution is capable of cleansing C. albicans biofilms on denture-base resin and has a persistent inhibitory effect on biofilm development, without any deteriorations of resin surface.
Databáze: OpenAIRE
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