Effect of New SNP Within Bovine Prolactin Gene Enhancer Region on Expression in the Pituitary Gland
| Autor: | Stanisław Kamiński, Anna Ruść, Krzysztof Flisikowski, Rafał R. Starzyński, Paweł Brym, E. Wójcik, Lech Zwierzchowski, Tadeusz Malewski |
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| Rok vydání: | 2007 |
| Předmět: |
Molecular Sequence Data
Gene Expression Single-nucleotide polymorphism Biology Polymerase Chain Reaction Polymorphism Single Nucleotide Biochemistry Sequence Homology Nucleic Acid Genotype Gene expression Genetics Animals RNA Messenger Enhancer Molecular Biology Transcription factor Polymorphism Single-Stranded Conformational Ecology Evolution Behavior and Systematics DNA Primers Base Sequence Promoter DNA General Medicine Molecular biology Prolactin Restriction enzyme Enhancer Elements Genetic Pituitary Gland Cattle |
| Zdroj: | Biochemical Genetics. 45:743-754 |
| ISSN: | 1573-4927 0006-2928 |
| Popis: | A new single nucleotide polymorphism was revealed using PCR-SSCP and sequencing methods within the bovine prolactin distal promoter region described as a functional enhancer. The A--G transition at position -1043 abolishes the recognition site for Hsp92II restriction endonuclease, allowing for PCR-RFLP genotyping. The application of real-time PCR revealed that the prolactin gene expression level in the pituitary was higher in cattle with the AA genotype than in those with the GG genotype. EMSA analysis, however, showed increased nuclear protein binding to the sequence variant with G, suggesting a possible inhibition event, in which the transcription factors Pit1, Oct1, and YY1 could be involved. |
| Databáze: | OpenAIRE |
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