Novel coding, translation, and gene expression of a replicating covalently closed circular RNA of 220 nt

Autor:	Ashkan Golshani, Srividhya Venkataraman, Bolin Liu, Mounir G. AbouHaidar, Tauqeer Ahmad
Rok vydání:	2014
Předmět:	Genetics Internal ribosome entry site Open reading frame Multidisciplinary Start codon Circular RNA Gene expression RNA Translation (biology) Biological Sciences Biology Virusoid
Zdroj:	Proceedings of the National Academy of Sciences. 111:14542-14547
ISSN:	1091-6490 0027-8424
Popis:	The highly structured (64% GC) covalently closed circular (CCC) RNA (220 nt) of the virusoid associated with rice yellow mottle virus codes for a 16-kDa highly basic protein using novel modalities for coding, translation, and gene expression. This CCC RNA is the smallest among all known viroids and virusoids and the only one that codes proteins. Its sequence possesses an internal ribosome entry site and is directly translated through two (or three) completely overlapping ORFs (shifting to a new reading frame at the end of each round). The initiation and termination codons overlap UGAUGA (underline highlights the initiation codon AUG within the combined initiation-termination sequence). Termination codons can be ignored to obtain larger read-through proteins. This circular RNA with no noncoding sequences is a unique natural supercompact "nanogenome."
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::954b0fa3fa5df9eda23bc265aea21ef5 https://doi.org/10.1073/pnas.1402814111 Zobrazit plný text záznamu