Anaphase DNA bridges induced by lack of RecQ5 inDrosophilasyncytial embryos
| Autor: | Katsumi Kawasaki, Haruna Sakurai, Fumiaki Ito, Misa Okado |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Anaphase bridge
Embryo Nonmammalian animal structures DNA repair Biophysics Mitosis Protein Serine-Threonine Kinases Giant Cells Biochemistry Nucleus chemistry.chemical_compound RecQ Structural Biology Genetics Animals Drosophila Proteins DNA Breaks Double-Stranded Molecular Biology Anaphase RecQ Helicases biology DNA Helicases DNA Cell Biology biology.organism_classification Molecular biology Checkpoint Kinase 2 enzymes and coenzymes (carbohydrates) Anaphase lag Drosophila melanogaster chemistry Double-strand break Centrosome Interphase |
| Zdroj: | FEBS Letters. 585:1923-1928 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2011.04.074 |
| Popis: | Drosophila melanogaster RecQ5, a member of the RecQ family, is expressed in early embryos. The loss of maternally-derived RecQ5 leads to spontaneous mitotic defects in syncytial embryos. We demonstrate that the mitotic defects are derived from anaphase DNA bridges. Pairs of daughter nuclei that had been linked by the bridges concurrently exited from the cycle and were eliminated by Chk2-dependent centrosome inactivation. These results suggest that the lack of RecQ5 leads to spontaneous double-stranded DNA breaks (DSBs). RecQ5 may function in the resolution of anaphase DNA bridges during mitosis or in DSB repair during interphase in syncytial Drosophila embryos. |
| Databáze: | OpenAIRE |
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