Phenotypic and fine genetic characterization of the D locus controlling fruit acidity in peach

Autor: K. Boudehri, Christelle Troadec, Abdelhafid Bendahmane, Annick Moing, Elisabeth Dirlewanger, Gaelle Cardinet
Přispěvatelé: Unité de recherches Espèces Fruitières et Vigne (UREFV), Institut National de la Recherche Agronomique (INRA), Unité de recherche en génomique végétale (URGV), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Station de physiologie végétale
Jazyk: angličtina
Rok vydání: 2009
Předmět:
0106 biological sciences
Chromosomes
Artificial
Bacterial
Plant Science
01 natural sciences
lcsh:Botany
Amplified Fragment Length Polymorphism Analysis
Genetics
0303 health sciences
education.field_of_study
Vegetal Biology
qtl
CARTOGRAPHIE GENETIQUE
LOCUS D
BIBLIOTHÈQUE BAC
ISOLEMENT DE GÈNE
adn
Chromosome Mapping
food and beverages
acidité
lcsh:QK1-989
Phenotype
Prunus
expression des gènes
Genetic Markers
DNA
Plant
Quantitative Trait Loci
Population
marqueur génétique
Locus (genetics)
fruit charnu
Quantitative trait locus
Biology
Genes
Plant
qualité organoleptique
03 medical and health sciences
Gene mapping
Genetic linkage
Research article
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
education
Gene
Gene Library
030304 developmental biology
locus
Genetic marker
Fruit
clonage
Amplified fragment length polymorphism
pêche
remaniement chromosomique
Biologie végétale
010606 plant biology & botany
Zdroj: BMC Plant Biology
BMC Plant Biology, 2009, 9 (59), 14 p. ⟨10.1186/1471-2229-9-59⟩
BMC Plant Biology, BioMed Central, 2009, 9 (59), 14 p. ⟨10.1186/1471-2229-9-59⟩
BMC Plant Biology 59 (9), 14 p.. (2009)
BMC Plant Biology, Vol 9, Iss 1, p 59 (2009)
ISSN: 1471-2229
Popis: BackgroundAcidity is an essential component of the organoleptic quality of fleshy fruits. However, in these fruits, the physiological and molecular mechanisms that control fruit acidity remain unclear. In peach theDlocus controls fruit acidity; low-acidity is determined by the dominant allele. Using a peach progeny of 208 F2trees, theDlocus was mapped to the proximal end of linkage group 5 and co-localized with major QTLs involved in the control of fruit pH, titratable acidity and organic acid concentration and small QTLs for sugar concentration. To investigate the molecular basis of fruit acidity in peach we initiated the map-based cloning of theDlocus.ResultsIn order to generate a high-resolution linkage map in the vicinity of theDlocus, 1,024 AFLP primer combinations were screened using DNA of bulked acid and low-acid segregants. We also screened a segregating population of 1,718 individuals for chromosomal recombination events linked to theDlocus and identified 308 individuals with recombination events close toD. Using these recombinant individuals we delimited theDlocus to a genetic interval of 0.4 cM. We also constructed a peach BAC library of 52,000 clones with a mean insert size of 90 kb. The screening of the BAC library with markers tightly linked toDlocus indicated that 1 cM corresponds to 250 kb at the vicinity of theDlocus.ConclusionIn the present work we presented the first high-resolution genetic map ofDlocus in peach. We also constructed a peach BAC library of approximately 15× genome equivalent. This fine genetic and physical characterization of theDlocus is the first step towards the isolation of the gene(s) underlying fruit acidity in peach.
Databáze: OpenAIRE
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