Direct Detection of Streptococcus suis from Cerebrospinal Fluid, Positive Hemoculture, and Simultaneous Differentiation of Serotypes 1, 1/2, 2, and 14 within Single Reaction
| Autor: | Sorasak Intorasoot, Ingyin Shun Lae Thu, Usanee Anukool, Chayada Sitthidet Tharinjaroen, Khajornsak Tragoolpua, Phadungkiat Khamnoi, Anusak Kerdsin |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Identification methods Serotype serotype 1 Streptococcus suis Point-of-care testing serotype 2 Article Cerebrospinal fluid serotype 1/2 Multiplex polymerase chain reaction medicine Immunology and Allergy Molecular Biology serotype 14 General Immunology and Microbiology biology multiplex PCR biology.organism_classification medicine.disease Virology Rapid disease progression serotyping Infectious Diseases Medicine direct detection Meningitis |
| Zdroj: | Pathogens, Vol 10, Iss 996, p 996 (2021) Pathogens Volume 10 Issue 8 |
| ISSN: | 2076-0817 |
| Popis: | Streptococcus suis is an emerging zoonotic bacterium causing septicemia and meningitis in humans. Due to rapid disease progression, high mortality rate, and many underdiagnosed cases by time-consuming routine identification methods, alternative diagnostic testing is essential. Among 29 broadly accepted S. suis serotypes, serotypes 2 and 14 are high prevalent however, many PCR assays showed an inability to differentiate serotype 2 from 1/2, and 1 from 14. In this study, we developed and validated a new multiplex PCR assay that facilitates the identification of only the 29 true serotypes of S. suis and simultaneously differentiates serotypes 1, 1/2, 2, and 14 within a single reaction. Importantly, the multiplex PCR could detect S. suis directly from positive hemocultures and CSF. The results revealed high sensitivity, specificity, and 100% accuracy with almost perfect agreement (κ = 1.0) compared to culture and serotyping methods. Direct detection enables a decrease in overall diagnosis time, rapid and efficient treatment, reduced fatality rates, and proficient disease control. This multiplex PCR offers a rapid, easy, and cost-effective method that can be applied in a routine laboratory. Furthermore, it is promising for developing point-of-care testing (POCT) for S. suis detection in the future. |
| Databáze: | OpenAIRE |
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