Determination of telmisartan in human blood plasma
| Autor: | Christel Hempen, Liane Gläsle-Schwarz, Uwe Karst, Ulrich Kunz |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Detection limit
Chemical ionization Analyte Chromatography medicine.diagnostic_test Human blood Chemistry Fluorescence spectrometry Angiotensin II receptor antagonist Mass spectrometry Biochemistry Fluorescence spectroscopy Analytical Chemistry Linear range Liquid chromatography–mass spectrometry Immunoassay Blood plasma medicine Environmental Chemistry Telmisartan Spectroscopy medicine.drug |
| Zdroj: | Analytica Chimica Acta. 560:35-40 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2005.12.036 |
| Popis: | A new liquid chromatography/atmospheric pressure chemical ionization-tandem mass spectrometry (LC/APCI-MS/MS) method with on-line sample clean-up for the determination of telmisartan in human blood plasma is presented. This technique is compared to a previously introduced enzyme-linked immunosorbent assay (ELISA), where fluorescence is used as detection method. For the LC/MS method applying an internal calibration via a deuterated internal standard, the limit of detection was 0.3 ng/mL, the limit of quantification was 0.9 ng/mL and the linear range extended from 0.9 to 1000 ng/mL. Forty-eight plasma samples from four healthy volunteers were analyzed in a pharmacokinetic study to obtain data for the method comparison. As a result, these two new and independent analytical methods for the determination of telmisartan in human blood plasma proved to yield comparable results for the amount of analyte. |
| Databáze: | OpenAIRE |
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