Characterization of the genetic structure of the brown trout (Salmo trutta) from 'Braduljica' Fish Farm, Serbia
| Autor: | Bozidar Raskovic, Radica Djedovic, Zoran Marković, Saša Marić, Nikola Molerovic, Ostojic Andric Dusica |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
General Mathematics
Fish farming rflp Zoology selection Introduced species Broodstock salmo trutta Aquatic organisms Brown trout pcr Salmo trutta 14. Life underwater Salmo lcsh:SF1-1100 selekcija biology Applied Mathematics CR mtDNA LDH nDNA Aquatic animal LDH nDNK biology.organism_classification ldh ndna PCR KR mtDNK cr mtdna Genetic structure RFLP lcsh:Animal culture |
| Zdroj: | Biotechnology in Animal Husbandry Biotechnology in Animal Husbandry (2019) 35(3):289-299 Biotechnology in Animal Husbandry, Vol 35, Iss 3, Pp 289-299 (2019) |
| ISSN: | 1450-9156 |
| Popis: | Due to the ecological concerns and preservation of genetic resources, the characterization of genetic structure of the brown trout (Salmo trutta) was carried out on the "Braduljica" fish farm. DNA was isolated from fin clips of 10 individuals, and after that molecular PCR-RFLP methods were used for distinguishing between Atlantic and Danubian lineages based on control region of the mitochondrial DNA (CR mtDNA) and lactate dehydrogenase gene of the nuclear DNA (LDH nDNA). Based on phenotypic characteristics it was estimated that out of 10 individuals included in this study five belonged to the allochtonous Atlantic lineage and remaining five belonged to the native Danubian lineage of brown trout. However, results of molecular analyses showed a high percentage of allochthonous genes among the individuals, which confirms the hybridization between these two lineages. Also, the results showed that the selection based on the phenotype is not adequate. In order to continue with proper broodstock management, it is necessary to eliminate allochtonous individuals of the Atlantic lineage from the broodstock. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|