IL-10 in vitro could enhance IFNγ expression and suppress PD-1 expression in CD8 T cells from esophageal cancer patients
| Autor: | Chong Li, Wenwei Zuo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male 0301 basic medicine Esophageal Neoplasms medicine.medical_treatment Programmed Cell Death 1 Receptor Stimulation CD8-Positive T-Lymphocytes Biology Flow cytometry Interferon-gamma 03 medical and health sciences 0302 clinical medicine Tumor Microenvironment medicine Humans Cytotoxic T cell Tumor microenvironment medicine.diagnostic_test Cell Biology Middle Aged Molecular biology In vitro Interleukin-10 Interleukin 10 030104 developmental biology Cytokine 030220 oncology & carcinogenesis Cytokines Female CD8 |
| Zdroj: | Experimental Cell Research. 379:159-165 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/j.yexcr.2019.03.038 |
| Popis: | IL-10 is commonly regarded as an immunoregulatory cytokine, but accumulating evidence suggests that IL-10 may promote CD8 T cell expansion and proliferation. In this study, tumor infiltrating (TI) and peripheral blood (PB) CD8 T cells were collected from esophageal cancer patients. Interestingly, IL-10 concentration in the tumor microenvironment increased with advancing tumor stage, while TI CD8 T cell-mediated IL-10 production decreased with advancing tumor stage. By flow cytometry, three distinctive subsets, including IL-10+IFNγ-, IL-10+IFNγ+, and IL-10-IFNγ+, could be observed in TI CD8 T cells. The former two subsets were present at much higher frequency in stage I and stage II patients than in stage III patients. IL-10+IFNγ+ TI CD8 T cells presented significantly higher IFNγ and lower PD-1 expression than the IL-10-IFNγ+ TI CD8 T cells. PB CD8 T cells, on the other hand, produced little IL-10 but potent IFNγ upon stimulation. Interestingly, intermediate level of exogenous IL-10 could significantly elevate the expression of IFNγ by PB CD8 T cells, while high level of exogenous IL-10 resulted in reduced expression of IFNγ by PB CD8 T cells. Exogenous IL-10 could not significantly reduce the frequencies of PD-1+ PB CD8 T cells, but significantly reduced the MFI of PD-1 in the PB CD8 T cells, especially in stage III patients. Together, this investigation demonstrated that IL-10 enhanced IFNγ expression and suppressed PD-1 expression in PB and TI CD8 T cells; however, the frequency of IL-10-expressing TI CD8 T cells decreased with increasing severity in esophageal cancer. |
| Databáze: | OpenAIRE |
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