Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolates
| Autor: | Kyung Hwa Yoo, Sun Mi Lee, Jeseung Oh, In Ho Park, Kyo Seok Lee, Jun Ho Song, Kook Jin Lim, Dongeun Yong, Myeonggil Han, Jeon Soo Shin |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.drug_class Aptamer Antibiotics lcsh:Medicine Antimicrobial susceptibility Biosensing Techniques Microbial Sensitivity Tests Electric Capacitance digestive system Article 03 medical and health sciences Reference test fluids and secretions 0302 clinical medicine polycyclic compounds Humans Antimicrobial stewardship Medicine lcsh:Science Multidisciplinary Bacteria business.industry lcsh:R Biological techniques Broth microdilution virus diseases Aptamers Nucleotide digestive system diseases Anti-Bacterial Agents 030104 developmental biology lcsh:Q business 030217 neurology & neurosurgery Biotechnology Biomedical engineering |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-70459-3 |
| Popis: | To prescribe effective antibiotics to patients with bacterial infections in a timely manner and to avoid the misuse of antibiotics, a rapid antimicrobial susceptibility test (AST) is essential. However, conventional AST methods require more than 16 h to provide results; thus, we developed an electrical AST (e-AST) system, which provides results within 6 h. The proposed e-AST is based on an array of 60 aptamer-functionalized capacitance sensors that are comparable to currently available AST panels and a pattern-matching algorithm. The performance of the e-AST was evaluated in comparison with that of broth microdilution as the reference test for clinical strains isolated from septic patients. A total of 4,554 tests using e-AST showed a categorical agreement of 97% with a minor error of 2.2%, major error of 0.38%, and very major error of 0.38%. We expect that the proposed e-AST could potentially aid antimicrobial stewardship efforts and lead to improved patient outcomes. |
| Databáze: | OpenAIRE |
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